Electrochemical Investigation on Kinetics of Xanthine Metabolism and Inhibition Effect of Febuxostat on Xanthine Oxidase Activity

Xanthine is a significant biomolecule and its concentration level in urine and blood plasma is an indicator of specified pathological states. Here, a new sensing platform was designed, which showed excellent analytical performance for xanthine. Importantly, it is the first time to investigate the kinetics of xanthine metabolic reaction by electrochemical method. The results demonstrated that the conversion of xanthine to uric acid completely conformed to the Michaelis-Menten kinetics. Furthermore, we also studied the inhibitory effect of febuxostat on xanthine oxidase activity detailed. As expected, the work may offer potential value for researchers in the treatment of hyperuricemia and gout.

Automated summary

Xanthine, a significant biomolecule, can be used as an indicator for specific pathological conditions. Researchers have developed a new sensing platform and used electrochemical method to study the kinetics of xanthine metabolic reaction for the first time. The results showed that the conversion of xanthine to uric acid followed Michaelis-Menten kinetics, and the inhibitory effect of febuxostat on xanthine oxidase activity was also studied.