Journal
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 245, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2022.114134
Keywords
Sodium butyrate; Thiram; Tibial dyschondroplasia; Wnt; ?-catenin; Gut microbial dysbiosis
Categories
Funding
- National Natural Science Foundation of China
- [31873031]
- [32172929]
Ask authors/readers for more resources
Thiram, a widely used pesticide, poses a threat to human safety and ecosystem stability. In this study, sodium butyrate was found to alleviate tibial dyschondroplasia induced by thiram exposure by regulating gene expression, promoting cell differentiation, and altering the gut microbial community.
Thiram is a dithiocarbamate pesticide widely used in agriculture as a fungicide for storing grains to prevent fungal diseases. However, its residues have threatened the safety of human beings and the stability of the ecosystem by causing different disease conditions, e.g., tibial dyschondroplasia (TD), which results in a sub-stantial economic loss for the poultry industry. So, the research on TD has a great concern for the industry and the overall GDP of a country. In current study, we investigated whether different concentrations (300, 500, and 700 mg/kg) of sodium butyrate alleviated TD induced under acute thiram exposure by regulating osteogenic gene expression, promoting chondrocyte differentiation, and altering the gut microbial community. According to the findings, sodium butyrate restored clinical symptoms in broilers, improved growth performance, bone density, angiogenesis, and chondrocyte morphology and arrangement. It could activate the signal transduction of the Wnt/beta-catenin pathway, regulate the expression of GSK-3 beta and beta-catenin, and further promote the production of osteogenic transcription factors Runx2 and OPN for restoration of lameness. In addition, the 16S rRNA sequencing revealed a significantly different community composition among the groups. The TD group increased the abundance of the harmful bacteria Proteobacteria, Subdoligranulum, and Erysipelatoclostridium. The sodium butyrate enriched many beneficial bacteria, such as Bacteroidetes, Verrucomicrobia, Faecalibacterium, Barnesiella, Rikenella, and Butyricicoccus, etc., especially at the concentration of 500 mg/kg. The mentioned concentration
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available