4.7 Article

Evaluation of di-rhamnolipid biosurfactants production by a novel Pseudomonas sp. S1WB: Optimization, characterization and effect on petroleum-hydrocarbon degradation

Journal

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
Volume 242, Issue -, Pages -

Publisher

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2022.113892

Keywords

Pseudomonas sp.; Di-rhamnolipids; Optimization; High stability; Petroleum-hydrocarbon pollutants; Biodegradation

Funding

  1. National Key Research and Devel- opment Program of China [2016YFC0503601]

Ask authors/readers for more resources

In this study, a novel strain of Pseudomonas sp. S1WB was isolated from an oil-contaminated water sample. The biosurfactants produced by this strain have the ability to reduce surface tension and emulsify, with optimal production achieved under specific conditions. The biosurfactants are highly stable and can degrade hydrocarbons effectively.
Rhamnolipid biosurfactants are multifunctional compounds that can play an indispensable role in biotechno-logical, biomedical, and environmental bioremediation-related fields, and have attracted significant attention in recent years. Herein, a novel strain Pseudomonas sp. S1WB was isolated from an oil-contaminated water sample. The biosurfactants produced by this strain have capabilities to reduce surface tension (SFT) at 32.75 +/- 1.63 mN/ m and emulsified 50.2 +/- 1.13 % in liquid media containing 1 % used engine oil (UEO) as the sole carbon source. However, the lowest SFT reduction (28.25 +/- 0.21), highest emulsification index (60.15 +/- 0.07), and the maximum yields (900 mg/L) were achieved under optimized conditions; where, the glucose/urea and glycerol/ urea combinations were found efficient carbon and nitrogen substrates for improved biosurfactants production. Biosurfactants product was characterized using ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) and detected various di-rhamnolipids congeners. In addition, the di-rhamnolipids produced by S1WB strain was found highly stable in terms of surface activity and EI indices at different environmental factors i.e. temperature, pH and various NaCl concentrations, where, emulsifying property was found high stable till 30 days of incubation. Moreover, the stain was capable to degrade hydrocarbon at 42.2 +/- 0.04 %, and the Gas chromatography-mass spectrometry (GC-MS) profile showed the majority of peak intensities of hydrocarbons have been completely degraded compared to control.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available