Journal
DOKLADY BIOCHEMISTRY AND BIOPHYSICS
Volume 506, Issue 1, Pages 210-214Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S1607672922050039
Keywords
Drosophila; Polycomb; PRE; repression of transcription; Su(Hw); CTCF; Pita; GAF; Psq
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Funding
- Russian Science Foundation [20-74-10099]
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Polycomb group (PcG) and Trithorax group (TrxG) proteins interact with Polycomb response elements (PRE) to regulate gene expression in Drosophila. The presence of architectural protein-binding sites induces the repressive activity of inactive PRE. This study shows that the recruitment of GAF and Psq to bxdPRE is dependent on the presence of adjacent binding sites for architectural proteins.
Polycomb group (PcG) repressors and Trithorax group (TrxG) activators of transcription are essential for the proper development and maintenance of gene expression profiles in multicellular organisms. In Drosophila, PcG/TrxG proteins interact with DNA elements called PRE (Polycomb response elements). We have previously shown that the repressive activity of inactive PRE in transgenes can be induced by architectural protein-binding sites. It was shown that the induction of repression is associated with the recruitment of PcG/TrxG proteins, including the DNA-binding factors Pho and Combgap. In the present study, we tested the association of the two other PRE DNA-binding factors, GAF and Psq, with bxdPRE in the presence and absence of sites for architectural proteins. As a result, it was shown that both factors can be efficiently recruited to the bxdPRE only in the presence of adjacent binding sites for architectural proteins Su(Hw), CTCF, or Pita.
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