Empirical estimation of genome-wide significance thresholds based on the 1000 Genomes Project data set

To assess the statistical significance of associations between variants and traits, genome-wide association studies (GWAS) should employ an appropriate threshold that accounts for the massive burden of multiple testing in the study. Although most studies in the current literature commonly set a genome-wide significance threshold at the level of P = 5.0 x 10(-8), the adequacy of this value for respective populations has not been fully investigated. To empirically estimate thresholds for different ancestral populations, we conducted GWAS simulations using the 1000 Genomes Phase 3 data set for Africans (AFR), Europeans (EUR), Admixed Americans (AMR), East Asians (EAS) and South Asians (SAS). The estimated empirical genome-wide significance thresholds were P-sig = 3.24 x 10(-8) (AFR), 9.26 x 10(-8) (EUR), 1.83 x 10(-7) (AMR), 1.61 x 10(-7) (EAS) and 9.46 x 10(-8) (SAS). We additionally conducted trans-ethnic meta-analyses across all populations (ALL) and all populations except for AFR (Delta AFR), which yielded P-sig = 3.25 x 10(-8) (ALL) and 4.20 x 10(-8) (Delta AFR). Our results indicate that the current threshold (P = 5.0 x 10(-8)) is overly stringent for all ancestral populations except for Africans; however, we should employ a more stringent threshold when conducting a meta-analysis, regardless of the presence of African samples.