Journal
DEVELOPMENT GENES AND EVOLUTION
Volume 232, Issue 5-6, Pages 115-123Publisher
SPRINGER
DOI: 10.1007/s00427-022-00696-1
Keywords
Gastrulation; Embryonic polarity; Planar cell polarity; Primitive streak elongation; Epithelial cell intercalation
Funding
- Wellcome Trust Investigator Award [107055/Z/15/Z]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2014R1A6A3A03053468]
- National Research Foundation of Korea [2014R1A6A3A03053468] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Wellcome Trust [107055/Z/15/Z] Funding Source: Wellcome Trust
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During primitive streak formation in the chick embryo, cVG1 and FGF signals play key roles. cVG1 is involved in primitive streak identity and elongation, while FGF signaling affects streak formation through regulation of cell intercalation.
During primitive streak formation in the chick embryo, cells undergo mesendoderm specification and convergent extension at the same time and in the same cells. Previous work has implicated cVG1 (GDF3) as a key factor for induction of primitive streak identity and positioning the primitive streak, whereas FGF signalling was implicated in regulating cell intercalation via regulation of components of the WNT-planar cell polarity (PCP) pathway. FGF has also been reported to be able to induce a primitive streak (but lacking the most axial derivatives such as notochord/prechordal mesendoderm). These signals emanate from different cell populations in the embryo, so how do they interact to ensure that the same cells undergo both cell intercalation and acquire primitive streak identity? Here we begin to address this question by examining in more detail the ability of the two classes of signals in regulating the two developmental events. Using misexpression of inducers and/or exposure to inhibitors and in situ hybridisation, we study how these two signals regulate expression of Brachyury (TBXT) and PRICKLE1 as markers for the primitive streak and the PCP, respectively. We find that both signals can induce both properties, but while FGF seems to be required for induction of the streak by cVG1, it is not necessary for induction of PRICKLE1. The results are consistent with cVG1 being a common regulator for both primitive streak identity and the initiation of convergent extension that leads to streak elongation.
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