Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
Volume 43, Issue -, Pages -Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbd.2022.101004
Keywords
Exosome; miRNAs; Cross-kingdom regulation; Microalgae; Pearl oyster
Funding
- National Natural Science Foundation of China [32002369]
- Special projects in key areas from the Department of Education of Guangdong Province [2020ZDZX1045]
- National Natural Science Foundation of Guangdong Province [2020A1515010691, 2019A1515011096]
- Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams, Department of Agriculture and Rural Affairs of Guangdong Province [2020KJ146]
- China Agriculture Research System of MOF and MARA
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This study found that miRNAs from plants can be transported to mollusks such as pearl oysters through the food chain, and they participate in various biological processes by targeting multiple genes. These findings provide new insights into the transmission of novel nutrients through the food chain.
MicroRNAs (miRNAs) can efficiently regulate gene expression at intracellular and extracellular levels. Plant derived miRNAs are highly enriched in animal haemolymph and regulate mammalian gene expression. However, evidence for food-derived miRNAs in Mollusca species is lacking. In this study, we fed the microalga Nannochloropsis oculata to the pearl oyster Pinctada fucata martensii and detected dietary miRNAs in exosomes isolated from the haemolymph by RNA-seq. In total, 273 endogenous miRNAs were identified in all biological replicates. We identified 23 microalgae-derived miRNAs in the exosomes of pearl oyster haemolymph. Most microalgae-derived miRNAs showed high expression levels in both exosomes and microalgae and exhibited apparent variation among individuals. These food-derived miRNAs were predicted to participate in endocytosis, apoptosis, signal transduction, energy metabolism, and biomineralization by targeting multiple genes. These findings demonstrated the cross-kingdom transport of miRNAs from microalgae to bivalves and provide insights into novel nutrient transmission through the food chain.
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