Silver nano/microparticle toxicity in the shrimp Litopenaeus vannamei (Boone, 1931)

The effects of silver nano/microparticles (AgP) on juvenile Litopenaeus vannamei shrimp were evaluated through several responses, aiming to use it as a prophylactic and therapeutic method. Shrimps (3.19 +/- 0.13 g) were exposed to clear water for 3 h with increasing concentrations of nanosilver (0; 25; 100; and 400 irg/l). After 3 h of exposure, they were transferred to water without nanosilver for 30 days (recovery). The weight gain and weekly growth were not affected by AgNP. Total antioxidant capacity (ACAP) increased in the hepatopancreas (exposure period) and gills (recovery) in shrimp exposed to AgNP. In muscle, ACAP was induced in shrimp exposed to 100 irg/l AgNP (exposure). In the gills, there was an increase in TBARS in shrimp exposed to 100 irg/l AgNP (re-covery). In the concentration of protein-associated sulfhydryl groups (P-SH), a decrease was observed in the hepatopancreas (recovery) in the 100 irg/l AgNP treatment. In chromaticity parameters, an increase in reddish tones was observed in shrimp exposed to 100 irg/l AgNP (recovery). An increase in granular hemocytes was verified in shrimp exposed to 25 and 400 irg/l AgNP during exposure. Tissues analyzed histologically showed normal patterns without apoptosis or necrosis processes, and after 30 d of recovery, only in one muscle sample of shrimp exposed to irg/l of AgNP was silver detected. It is concluded that a prophylactic action of short duration (3 h) mostly did not affected the welfare of shrimp L. vannamei and can be considered its use as a therapeutic strategy.

Automated summary

The effects of silver nano/microparticles on juvenile L. vannamei shrimp were evaluated. The study found that short-term exposure to nanosilver did not affect the growth and welfare of the shrimp. However, it did induce changes in antioxidant capacity, chromaticity parameters, and hemocyte count in certain tissues. Overall, the use of silver nanoparticles as a prophylactic and therapeutic method for shrimp shows promise.