Toxicity evaluation of chlorpyrifos and its main metabolite 3,5,6-trichlor- o-2-pyridinol (TCP) to Eisenia fetida in different soils

The present study utilized a biomarker response method to evaluate the effect of 3,5,6-trichloro-2-pyridinol (TCP) in artificial and natural soils on Eisenia fetida after 7, 14, 28, 42 and 56 days exposure. Results indicated that TCP induced excessive reactive oxygen species, caused oxidative stress and DNA damage to Eisenia fetida. Biomarker responses were standardized to calculate the Integrated Biomarker Response (IBR) index. The IBR index of three enzymes (superoxide dismutase, catalase and glutathione S-transferase) activities showed that TCP induced the oxidative stress to E. fetida in red clay was stronger than in the other three soils. Specifically, chlorpyrifos exposure group showed a lower toxicity than TCP exposure group after 28 days exposure but a higher toxicity than TCP exposure group after 56 days exposure. Despite the deficiencies of this study, the above information is of great significance for assessing the risk of chlorpyrifos and its metabolite TCP pollution in soil ecosystems.

Automated summary

This study evaluated the effect of TCP on Eisenia fetida using a biomarker response method and found that TCP induced oxidative stress and DNA damage. The results showed that TCP induced stronger oxidative stress to E. fetida in red clay compared to other soils. The toxicity of the chlorpyrifos exposure group also varied after different durations of exposure.