Hybrid perylene-cored poly(amidoamine) dendrimer with coumarin and calcozine red 6G end groups: From photophysical properties to cell imaging

In this article, we looked at how to make fluorescence hybrid dendrimers using perylenediimide (PTCDI) cores and calcozine red 6 G and coumarin end groups. Perylenetetracarboxylic dianhydride (PTCDA) is used as core, and 4th generation poly(amidoamine) dendrimer (PAMAM) is synthesized to produce PG4.0. Then, PG4.0 is reacted with calcozine red 6 G and 7-methacryloyloxy-4-methylcoumarin (MCMC). Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance (1H NMR), X-ray diffraction (XRD), and field emission scanning electron microscopy (FE-SEM) are used to characterize the structure of synthesized fluorescent hybrid dendrimers. Optical properties are demonstrated using fluorescence microscopy, a fluorescence spectropho-tometer, and UV-vis-NIR reflectance spectra. Hybrid dendrimers were transparent in the NIR range, according to UV-Vis-NIR reflectance spectra. Moreover, quantum yield (Phi s) of hybrid dendrimers was calculated in dime-thylformamide (DMF), ethanol, and distilled water (H2O). PG4R32 and PG4M16R16 showed the maximum quantum yield in ethanol due to great interaction of structure with ethanol and the arrangement of ring-opened amide shape of calcozine red 6 G gather in compound. At long last, SH-SY5Y cells were utilized to measure cell uptake and fluorescence imaging exhibitions of PG4.0 (perylene-3,4,9,10-tetracarboxylic diimide (PTCDI)-based generation 4 amino terminated poly(amidoamine)dendrimers), PG4M32 (perylene-3,4,9,10-tetracarboxylic dii-mide (PTCDI)-based generation 4 amino terminated poly (amidoamine) dendrimers in which 32 amine groups of PAMAM are conjugated with coumarin derivatives), PG4R32 (perylene-3,4,9,10-tetracarboxylic diimide (PTCDI)-based generation 4 amino terminated poly(amidoamine)dendrimers in which 32 amine groups of PAMAM are conjugated with rhodamine derivatives), and PG4M16R16 (perylene-3,4,9,10-tetracarboxylic dii-mide (PTCDI)-based generation 4 amino terminated poly(amidoamine)dendrimers in which 16 amine groups of PAMAM are conjugated with rhodamine and coumarin derivatives) pigments, and as a result, pigments had a great capacity for live-cell fluorescence imaging.

Automated summary

This article focuses on the synthesis and characterization of fluorescence hybrid dendrimers using perylenediimide cores and calcozine red 6 G and coumarin end groups. The optical properties and cell uptake of the synthesized dendrimers are also investigated.