Fibroblast GSK-3α Promotes Fibrosis via RAF-MEK-ERK Pathway in the Injured Heart

Background: Heart failure is the leading cause of mortality, morbidity, and health care expenditures worldwide. Numerous studies have implicated GSK-3 (glycogen synthase kinase-3) as a promising therapeutic target for cardiovascular diseases. GSK-3 isoforms seem to play overlapping, unique and even opposing functions in the heart. Previously, we have shown that of the 2 isoforms of GSK-3, cardiac fibroblast GSK-3 beta acts as a negative regulator of myocardial fibrosis in the ischemic heart. However, the role of cardiac fibroblast-GSK-3 alpha in the pathogenesis of cardiac diseases is completely unknown. Methods: To define the role of cardiac fibroblast-GSK-3 alpha in myocardial fibrosis and heart failure, GSK-3 alpha was deleted from fibroblasts or myofibroblasts with tamoxifen-inducible Tcf21- or Postn-promoter-driven Cre recombinase. Control and GSK-3 alpha KO mice were subjected to cardiac injury and heart parameters were evaluated. The fibroblast kinome mapping was carried out to delineate molecular mechanism followed by in vivo and in vitro analysis. Results: Fibroblast-specific GSK-3 alpha deletion restricted fibrotic remodeling and preserved function of the injured heart. We observed reductions in cell migration, collagen gel contraction, alpha-SMA protein levels, and expression of ECM genes in TGF beta 1-treated KO fibroblasts, indicating that GSK-3 alpha is required for myofibroblast transformation. Surprisingly, GSK-3 alpha deletion did not affect SMAD3 activation, suggesting the profibrotic role of GSK-3 alpha is SMAD3 independent. The molecular studies confirmed decreased ERK signaling in GSK-3 alpha-KO CFs. Conversely, adenovirus-mediated expression of a constitutively active form of GSK-3 alpha (Ad-GSK-3 alpha(S21A)) in fibroblasts increased ERK activation and expression of fibrogenic proteins. Importantly, this effect was abolished by ERK inhibition. Conclusions: GSK-3 alpha-mediated MEK-ERK activation is a critical profibrotic signaling circuit in the injured heart, which operates independently of the canonical TGF-beta 1-SMAD3 pathway. Therefore, strategies to inhibit the GSK-3 alpha-MEK-ERK signaling circuit could prevent adverse fibrosis in diseased hearts.

Automated summary

This study reveals the important role of cardiac fibroblast-GSK-3 alpha in myocardial fibrosis and heart failure, and identifies the molecular mechanism underlying this role through fibroblast kinome mapping. The results suggest that GSK-3 alpha plays a critical role in fibroblast transformation and fibrotic remodeling, independently of the canonical TGF-beta 1-SMAD3 pathway.