Effects of phthalate esters on soil microbial community under different planting patterns in Northern China: Case study of Hebei Province

Soil microorganisms are biological factors involved in the farmland environment. The factors that shape soil microbial communities and how these are influenced by geographic location, planting pattern (open-field or greenhouse), and soil organic pollutants (phthalate esters, PAEs) remain poorly understood at large scales. Using 16 S rRNA gene and ITS sequencing, we characterized the soil microbiota in open-field and greenhouse soils in Hebei Province, China, and correlated their structure and composition to geographic location, planting pattern and PAEs. Compared with geographic location, planting pattern is more decisive for shaping soil microbes and has more significant effects on bacteria, and the effects are shaped by the number and types of core OTUs. PAEs participated in the shaping of soil microbial communities by altering the relative abundances of dominant mi-croorganisms in the two planting patterns, and the effects of PAEs with high Kow were more significant. PAEs have a greater impact on bacteria than fungi in both planting patterns. Bacteria in the greenhouse soil were sensitive to the 9 kinds of PAEs detected, however in the open-field samples, mainly responded to PAEs with high Kow and rarely respond to PAEs with low Kow. DEHP and DBP, as two monomers with the highest concentration, have significant effects on dominant genera of microorganisms under both planting patterns, with inhibiting effect on bacteria and significantly promotion on fungi. Our study clarified the factors that have a substantial impact on soil microorganisms at the provincial scale and the mechanisms involved in shaping soil microbial community structure, as well as the significant impact of PAEs on soil microbial dominant microorganisms.

Automated summary

This study characterized the soil microbiota in open-field and greenhouse soils in Hebei Province, China, and found that planting pattern has a more decisive effect on shaping soil microbes than geographic location. The presence of PAEs, especially those with high Kow values, significantly influenced soil microbial communities in different planting patterns.