Journal
CHEMOSPHERE
Volume 303, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.chemosphere.2022.135074
Keywords
Antihypertensive drugs; Amlodipine; Telmisartan; Hydrochlorothiazide; HPLC; Spectrophotometry
Categories
Funding
- Scientific Research Projects Com-mission of Ankara University [21B0237005and 21H0237005]
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A novel RP-HPLC method was developed for the quantification of TLM and HYD in a ternary mixture of synthetic laboratory mixture. A new spectrophotometric method was also applied for simultaneous determination of active substances.
For the quantifiable amounts of Telmisartan (TLM) and Hydrochlorothiazide (HYD) in the presence of Amlodipine (AML) in a ternary mixture of synthetic laboratory mixture, a novel, sensitive, quick, and practical reversed-phase high-performance liquid chromatography (RP-HPLC) method was given. In order to separate, a Waters Spherisorb ODS-2 C18 column was used. For HYD, TLM, and AML, these techniques were viable over linearity ranges of 4-12 mu g/mL, 4-25 mu g/mL, and 5-40 mu g/mL, respectively. The mobile phase system was acetonitrile:methanol: phosphate buffer at pH 2.5 (65:5:30 v/v/v), and the flow rate was 1.5 mL/min. Novel spectrophotometric methods were applied for active substances to determine simultaneously. The first method is absorptivity centering using factorized spectrum, and the second method is dual amplitude difference coupled
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