4.5 Article

The Volatile Profiles and DNA Barcodes of Lauraceae Species from the Ocotea Complex with Occurrence in the Brazilian Amazon

Journal

CHEMISTRY & BIODIVERSITY
Volume 19, Issue 10, Pages -

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbdv.202200337

Keywords

DNA barcode; ITS; phenylpropanoids; statistical multivariate analysis; terpenoids

Funding

  1. National Council for Scientific and Technological Development (CNPq, Brazilian Government)

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This study classified the Ocotea complex species in Para state, Brazil based on their chemical and molecular markers, including essential oil compositions and DNA sequences. The results showed that these species can be divided into two main groups.
The Ocotea complex accommodates most of the taxonomic diversity of Neotropical Lauraceae with economic importance and biological potential attributed to their essential oils (EOs) and extracts. However, the botanical taxonomy has had limitations due to the difficulty of identifying and delimiting species and genera. The chemical and molecular markers of Ocotea complex species in Para state, Brazil, were assessed according to their EO compositions and DNA sequences of matK, trnL-trnF, and ITS regions. The multivariate analysis of EOs constituents has classified them into two main clusters characterized by oils rich in (I) terpenoids and phenylpropanoids and (II) sesquiterpenes. We conducted a phylogenetic analysis of species based on DNA barcode sequences on the Bayesian Inference (PP: 0.70-1,0) and Maximum Likelihood (BS: 72-100 %). The comparison between the volatile profiles and phylogenetic data indicates two main groups for these species collected from the Ocotea complex.

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