Journal
CHEMIE INGENIEUR TECHNIK
Volume 94, Issue 11, Pages 1836-1844Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cite.202200062
Keywords
Biocatalysis; Chiral amines; Enzyme discovery; Function prediction; Transaminase
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Funding
- German Research Foundation (DFG) [Bo1862/16-1, Ho4754/4-1]
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This study demonstrates that the activity level of amine transaminases (ATAs) towards a specific substrate can be predicted solely from the sequence, with hydrophobic side chains being a better predictor than sequence identity. Additionally, four out of the 15 ATAs showed good operational stability.
Amine transaminases (ATAs) are biocatalysts for the synthesis of chiral amines and can be identified in sequence databases by specific sequence motifs. This study shows that the activity level towards the model substrate 1-phenylethylamine can be predicted solely from the sequence. To demonstrate this, 15 putative ATAs with a different distribution of hydrophobic or hydrophilic amino acid side chains near the active site were characterized. Hydrophobic side chains were associated with a high activity level and were a better predictor of activity than global sequence identity to known ATAs with high or low activities. Enzyme stability investigations revealed that four out of the 15 ATAs showed a good operational stability.
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