Journal
CHEMIE INGENIEUR TECHNIK
Volume 94, Issue 11, Pages 1853-1859Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cite.202200084
Keywords
ABTS; Activity screening; Laccase; Unspecific peroxygenase; Wild-type fungi
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Funding
- Projekt DEAL
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This study presents a new screening strategy based on solid cultivation of wild-type fungi to identify novel unspecific peroxygenases (UPOs). The identification of a promising candidate strain showcases the applicability of this approach.
Biocatalytic oxidation reactions are in high demand. Among the applied enzymes, the heme-thiolate enzyme subfamily of unspecific peroxygenases (UPOs), which relies on hydrogen peroxide as cosubstrate and oxidant, has generated great interest. Almost two decades after their first description, databases provide thousands of putative UPO sequences, but only a few enzymes have been characterized. To address this gap, efficient screening methods for the identification of novel UPOs are necessary. Here, a new screening strategy is presented based on solid cultivation of wild-type fungi. The identification of a promising candidate strain highlights the applicability of this approach.
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