Journal
CHEMIE INGENIEUR TECHNIK
Volume 94, Issue 12, Pages 1977-1984Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cite.202200097
Keywords
Chinese hamster ovary cells; Monoclonal antibody; N-1 perfusion; Ultra-high cell density
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This study established a one-step inoculum production method based on an ultra-high cell density working cell bank, which can effectively improve the efficiency of producing biopharmaceuticals and increase manufacturing capacity.
Various approaches to process intensification are currently being investigated to ensure time and cost savings when producing biopharmaceuticals. In the present study, a one-step inoculum production was established based on an ultra-high cell density working cell bank with immunoglobulin G producing Chinese hamster ovary cells. Cryovials were used for direct inoculation of 1-L wave-mixed perfusion bioreactors. When reaching around 180 center dot 10(6) cells mL(-1) in N-1 perfusion mode, low-seed and high-seed fed-batch experiments in shake flasks were inoculated, as was the case for 250-mL stirred single-use bioreactors. Additionally, proof-of-concept runs at 50-L and 200-L scale were successfully performed. The intensification approach presented allows manufacturing capacity to be increased by up to 50 %.
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