Preparation of Cerium Dioxide Functionalized Magnetic Layered Double Hydroxides for High-efficiency Phosphopeptide Enrichment

In this work, we prepared a material with magnetic nanoparticles (Fe3O4) as core, layered double hydroxides(LDHs) as affinity shell, and cerium dioxide(CeO2) as functional molecules(denoted as Fe3O4@LDH-CeO2). On the basis of combined immobilized metal ion affinity chromatography(IMAC) and metal oxide affinity chromatography(MOAC), Fe3O4@LDH-CeO2 was used to enrich phosphopeptides with high efficiency. The material exhibited high selectivity(alpha-casein:beta-casein:BSA=1:1:5000, mass ratio), high recovery(95.87%), and good reusability of 10 times adsorption-desorption experiments. The feasibility of Fe3O4@LDH-CeO2 was further investigated by extracting phosphopeptides from biological samples(nonfat milk, serum, saliva, and A549 cell lysate).

Automated summary

In this study, Fe3O4@LDH-CeO2 material was prepared and demonstrated to efficiently enrich phosphopeptides in solid-phase extraction. The material exhibited high selectivity, high recovery, and good reusability, making it a promising tool for phosphopeptide extraction from biological samples.