Journal
CHEMICAL PAPERS
Volume 77, Issue 2, Pages 987-1003Publisher
SPRINGER INT PUBL AG
DOI: 10.1007/s11696-022-02530-4
Keywords
Molecularly imprinted polymers (MIP); Porcine dsDNA; Pyrrole; Titanium dioxide; Nanocomposite film; Biosensor
Categories
Ask authors/readers for more resources
In this study, novel molecularly imprinted polymer nanocomposite films were developed for detecting porcine double-stranded deoxyribonucleic acid (dsDNA) in food samples. The nanocomposite films showed excellent binding capacity, recognition selectivity, and reusability, making them suitable for porcine identification.
In the present study, novel molecularly imprinted polymer (MIP) nanocomposite films were prepared for the detection of porcine double-stranded deoxyribonucleic acid (dsDNA) in food samples. A fragment of dsDNA from the pork mitochondrial genome was chosen as a template molecule for porcine identification. The molecularly imprinted polymer (MIP) nanocomposite films of polypyrrole-TiO2 (PPy/TiO2) were synthesized electrochemically on the surface of indium-tin-oxide (ITO) glass electrode. A strong voltammetric (DPV) response was observed for capturing dsDNA from the samples containing porcine. The batch rebinding experiments revealed that PPy/TiO2 MIP possessed excellent binding capacity (Q = 87.44%) and a high imprint factor (2.30) than non-imprinted polymers (NIPs). The selectivity experiments indicated that MIP had excellent recognition selectivity for porcine dsDNA. MIP exhibited higher relative selectivity coefficient (K ' = 4.26 mu M/mu L) toward porcine dsDNA as compared to structural analogue beef dsDNA (K ' = 0.23 mu M/mu L) and chicken dsDNA (K ' = 0.21 mu M/mu L). This could be attributed to the introduction of the imprinted cavities exactly matching the porcine dsDNA on the surface of MIP nanocomposite film. Furthermore, PPy/TiO2 MIP showed a high reusability and low LOD (2.06 mu M) and LOQ (6.89 mu M) values.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available