4.4 Article

N-Glycan Engineering: Constructing the N-GlcNAc Stump

Journal

CHEMBIOCHEM
Volume -, Issue -, Pages -

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.202200388

Keywords

endoglycosidases; glycoproteins; N-acetylglucosamines; N-glycosylation; transglycosylation

Funding

  1. National Natural Science Foundation of China [21807061, 91953111]
  2. Natural Science Foundation of Tianjin [18JCYBJC41600]

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This article briefly introduces the methods for generating N-glycosylated proteins and peptides through endoglycosidase-catalyzed reactions and highlights the current challenges and opportunities in the scientific community.
N-Glycosylation is often essential for the structure and function of proteins. However, N-glycosylated proteins from natural sources exhibit considerable heterogeneity in the appended oligosaccharides, bringing daunting challenges to corresponding basic research and therapeutic applications. To address this issue, various synthetic, enzymatic, and chemoenzymatic approaches have been elegantly designed. Utilizing the endoglycosidase-catalyzed transglycosylation method, a single N-acetylglucosamine (N-GlcNAc, analogous to a tree stump) on proteins can be converted to various homogeneous N-glycosylated forms, thereby becoming the focus of research efforts. In this concept article, we briefly introduce the methods that allow the generation of N-GlcNAc and its close analogues on proteins and peptides and highlight the current challenges and opportunities the scientific community is facing.

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