Structural and mechanical anisotropy in rheotactically aligned bacterial cellulose

In this work, we demonstrate the preparation of oriented bacterial cellulose from Komagataeibacter sucrofermentans by rheotactic growth in a simple and adaptable setup. The resulting materials were assessed by their yields, geometric densities, and by X-ray diffraction, scanning electron and optical microscopy, and mechanical testing. They exhibited large differences in toughness, resulting from differences in fracture strain or highly anisotropic strengths. Their growth characteristics, structural and mechanical anisotropies and crystalline phase characteristics are discussed and compared to statically grown references and to instances from the literature. Here, we consider the length scales of structural anisotropy in native bacterial cellulose pellicles, and the origin of mechanical anisotropy. Further, we identify a tentative limit on achievable structural alignment in bacterial cellulose, as well as a correlation between crystallinity and disorder in the crystalline phase of bacterial cellulose.

Automated summary

In this study, oriented bacterial cellulose was prepared from Komagataeibacter sucrofermentans through rheotactic growth in a simple and adaptable setup. The resulting materials were evaluated for their properties, showing differences in toughness due to structural and mechanical anisotropies. This work also discusses the limits of achievable structural alignment in bacterial cellulose and the correlation between crystallinity and disorder in the crystalline phase.