CD34+ cell atlas of main organs implicates its impact on fibrosis

Rationale CD34(+) cells are believed being progenitors that may be used to treat cardiovascular disease. However, the exact identity and the role of CD34(+) cells in physiological and pathological conditions remain unclear. Methods We performed single-cell RNA sequencing analysis to provide a cell atlas of normal tissue/organ and pathological conditions. Furthermore, a genetic lineage tracing mouse model was used to investigate the role of CD34(+) cells in angiogenesis and organ fibrosis. Results Single-cell RNA sequencing analysis revealed a heterogeneous population of CD34(+) cells in both physiological and pathological conditions. Using a genetic lineage tracing mouse model, we showed that CD34(+) cells not only acquired endothelial cell fate involved in angiogenesis, but also, CD34(+) cells expressing Pi16 may transform into myofibroblast and thus participate in organ fibrosis. Conclusion A heterogeneous CD34(+) cells serve as a contributor not only to endothelial regeneration but also a wound healing response that may provide therapeutic insights into fibrosis.

Automated summary

This study provides insights into the heterogeneous nature and functions of CD34(+) cells in both physiological and pathological conditions, highlighting their involvement in angiogenesis and organ fibrosis.