Journal
CARBOHYDRATE POLYMERS
Volume 294, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2022.119810
Keywords
Hydrogel; Alginate; Restricted access material; HPLC; Microextraction; Human plasma
Categories
Funding
- CAPES (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior)
- CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico)
Ask authors/readers for more resources
A novel alginate-based hydrogel device was successfully prepared and applied for solid-phase microextraction of drugs in human plasma, showing high extraction efficiency and precision. This concept has not been explored in previous literature.
A novel lab-made alginate-based hydrogel device was successfully prepared and applied as a sorption material for the solid-phase microextraction of drugs (fluoxetine and its metabolite, norfluoxetine) in human plasma, with subsequent determination by high performance liquid chromatography-fluorescence detection (HPLC-FD). When supported in a polypropylene hollow fiber, the alginate was able to extract the analytes and functioned as a restricted access material, excluding >95 % of proteins from the biological matrix. The results indicate the potential use of this phase/device for quantitative drugs extraction from biological matrices at concentrations compatible with those typical in the literature (0.5 mu g mL-1), and with satisfactory precision (13.4 % for fluoxetine and 6.2 % for norfluoxetine). Such outcomes, promoted by a simple and inexpensive material, open a new perspective of exploration of hydrogels as the sorption phase in biological matrices, a concept previously unexplored in the literature.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available