Mesona chinensis polysaccharide/zein nanoparticles to improve the bioaccesibility and in vitro bioactivities of curcumin

The aim of this study was to enhance the stability, bioaccesibility, and bioactivities of curcumin using a com-posite nanoparticle prepared from zein and Mesona chinensis polysaccharide (MCP). Curcumin-loaded nano-particles (ZMC NPs) were prepared. ZMC NPs showed smooth spherical structure with a high encapsulation efficiency (94 %), a small average particle size (223 nm), and surface negative charge (-34.53 mV). Compared with free curcumin, encapsulated curcumin has been shown to have better environmental stability, higher antioxidant activity and bioaccesibility. The surface coating of zein NPs with MCP makes them more amenable to uptake by intestinal epithelial cells. Studies on in vitro antitumor activity of ZMC NPs showed that they could enter hepatocellular carcinoma cells, induce cell apoptosis, promote ROS production, and alter mitochondrial membrane potential, showing higher in vitro antitumor activity compared to free curcumin. These results may contribute to the development of novel curcumin oral delivery systems.

Automated summary

This study aimed to enhance the stability, bioaccessible, and bioactivities of curcumin by preparing composite nanoparticles using zein and Mesona chinensis polysaccharide (MCP). The prepared curcumin-loaded nanoparticles (ZMC NPs) exhibited a smooth spherical structure with high encapsulation efficiency, small particle size, and negative surface charge. Encapsulated curcumin showed better environmental stability, higher antioxidant activity, and bioaccesibility compared to free curcumin. The surface coating with MCP improved the uptake of ZMC NPs by intestinal epithelial cells. In vitro studies demonstrated that ZMC NPs had higher antitumor activity, inducing cell apoptosis, promoting ROS production, and altering mitochondrial membrane potential in hepatocellular carcinoma cells. These findings are significant for the development of novel curcumin oral delivery systems.