Journal
CARBOHYDRATE POLYMERS
Volume 294, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2022.119777
Keywords
Calcium oxalate; Fructan; Aspidopterys obcordate; Anti-urolithiasis; Fruit fly
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Funding
- One Belt and One Road Ethnic Medicine Research and Industry Cooperation of the Cooperation Bureau of Chi-nese Academy of Sciences [Y6ZK131B01]
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This study identified a 2.3 kD fructan from Aspidopterys obcordata vine with unique anti-urolithiasis activity, reducing the size of calcium oxalate crystals through multiple mechanisms.
Aspidopterys obcordata vine is a Chinese Dai ethnic herb used to treat urolithiasis. However, the material basis and underlying mechanisms remain undefined. In this study, a 2.3 kD inulin-like A. obcordata fructan (AOFOS) was isolated by size exclusion column chromatography and characterized by ultrahigh-performance liquid chromatography-ion trap-time of flight mass spectrometry (UPLC-IT-TOF-MS), nuclear magnetic resonance (NMR) spectroscopy, gas chromatography mass spectrometry (GC-MS) and high-performance gel permeation chromatography (HGPC). In addition, AOFOS showed unique anti-urolithiasis activity in Drosophila kidney stone models. Mechanism study indicated that AOFOS reduced the size of calcium oxalate crystals by inhibiting the formation of large size crystals and the generation rate of calcium oxalate crystals as well as the crystal form conversion from calcium oxalate monohydrate (COM) to calcium oxalate dihydrate (COD).
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