Journal
BRAIN RESEARCH
Volume 1790, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.brainres.2022.147984
Keywords
Caffeine; Medial Parabrachial Nucleus; Adenosine A1 receptors; Synaptic transmission; Apnea neonatorum; Patch-clamp
Categories
Funding
- Zhejiang Provincial Natural Science Foundation [Y17H010022]
- Scientific Research Foundation of Health Bureau of Zhejiang Province [2018ZD010]
- Wenzhou City Science and Technology Bureau Grant [Y20170133]
- Natural Science Foundation of China [81870073]
- 2018 Clinical Research Foundation of the 2nd Affiliated Hospital of Wenzhou Medical University [SAHoWMUCR2018-06-220]
- Project of Key Innovative Disciplines of Children Sleep Medicine of Zhejiang
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Caffeine, a widely used first-line drug for treating neonatal apnea, has been found to excite the neurons in the medial parabrachial nucleus (MPB) by blocking A1R receptors. This study suggests that A1Rs in MPB may be potential targets for caffeine in reducing apnea neonatorum.
Caffeine has been used as a first-line drug for treatment of apnea neonatorum for decades due to its high safety and effectiveness. Studies report that caffeine mainly acts as a blocker of Adenosine Receptors (ARs). However, the mechanism of caffeine in reducing apnea neonatorum in the central nervous system has not been fully explored. Medial parabrachial nucleus (MPB) is part of the respiratory center of the pons that may be related to the activity of caffeine. Previous studies have not explored the effect and mechanism of caffeine on MPB neurons. To elucidate this, the current study used antagonists of A1 and A2a receptors to mimic the effect of caffeine in MPB of mice in vitro using the patch-clamp technique. The firing rates and spontaneous post-synaptic currents were recorded. The findings of the study showed that caffeine excited MPB neurons. Notably, the adenosine A1R antagonist 8-cyclopentyl-1,3-dimethyl-xanthine (CPT) but not the adenosine A2aR antagonist Istradefylline (KW6002) mimicked the exciting effect of caffeine, implying that caffeine excited MPB neurons in mice by blocking AlRs. Further, the results indicated that caffeine could increase efficiency of synaptic transmission to excite MPB neurons. These findings suggest that A1Rs in MPB may be potential targets for caffeine in reducing apnea neonatorum.
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