Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 120, Issue 1, Pages 22-40Publisher
WILEY
DOI: 10.1002/bit.28246
Keywords
biocatalyst; biosensor; electrospinning; enzyme immobilization; nanofiber; polymer
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This review article systematically discusses the recent trends and applications of enzyme immobilization using natural polymers to prepare nanofibers. Nanostructured supports have been proven to exhibit better catalytic performance and immobilization efficiency.
All the disciplines of science, especially biotechnology, have given continuous attention to the area of enzyme immobilization. However, the structural support made by material science intervention determines the performance of immobilized enzymes. Studies have proven that nanostructured supports can maintain better catalytic performance and improve immobilization efficiency. The recent trends in the application of nanofibers using natural polymers for enzyme immobilization have been addressed in this review article. A comprehensive survey about the immobilization strategies and their characteristics are highlighted. The natural polymers, e.g., chitin, chitosan, silk fibroin, gelatin, cellulose, and their blends with other synthetic polymers capable of immobilizing enzymes in their 1D nanofibrous form, are discussed. The multiple applications of enzymes immobilized on nanofibers in biocatalysis, biosensors, biofuels, antifouling, regenerative medicine, biomolecule degradation, etc.; some of these are discussed in this review article.
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