Journal
BIOSENSORS & BIOELECTRONICS
Volume 214, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2022.114487
Keywords
Circulating biomarkers; Exosome; Microfluidic; Aptamer; Lung cancer detection
Categories
Funding
- Start-up Foundation of Guangzhou Laboratory [YW-JCZD0101]
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The study introduces an automated centrifugal microfluidic disc system combined with a novel aptamer fluorescence system to efficiently isolate and purify exosomes for detecting cancer with high accuracy in clinical samples. Compared to traditional techniques, this method is cost-effective, rapid, high in purity, sensitivity, and specificity.
Non-invasive methods of detecting cancer by circulating exosomes are challenged by inefficient purification and identification. This study hereby proposed an automated centrifugal microfluidic disc system combined with functionalized membranes (Exo-CMDS) to isolate and enrich exosomes, which will then be processed by a novel aptamer fluorescence system (Exo-AFS) in order to detect the exosome surface proteins in an effective manner. Exo-CMDS features in highly qualified yields with optimal exosomal concentration of 5.1 x 109 particles/mL from trace amount of blood samples (<300 mu L) in only 8 min, which truly accomplishes the exosome isolation and purification in one-step methods. Meanwhile, the limit of detection (LOD) of PD-L1 in Exo-AFS reaches as low as 1.58 x 105 particles/mL. In the trial of clinical samples, the diagnostic accuracy of lung cancer achieves 91% (95% CI: 79%-96%) in contrast to the exosome ELISA (area under the curve: 0.9378 versus 0.8733; 30 patients). Exo-CMDS and Exo-AFS display the precedence in the aspects of inexpensiveness, celerity, purity, sensitivity and specificity when compared with the traditional techniques. Such assays potentially grant a practicable way of detecting inchoate cancers and guiding immunotherapy in clinic.
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