Journal
BIOSENSORS & BIOELECTRONICS
Volume 215, Issue -, Pages -Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2022.114605
Keywords
Carboxylated Ru(II) complexes; Dual-amplification strategy; Iron-based metal-organic frameworks; Neuron-specific enolase
Categories
Funding
- National Natural Science Founda- tion of China
- Applied Basic Research Foundation of Yunnan Province
- Natural Science Foundation of Shandong Province
- Program for Scientific Research Innovation Team in Colleges and Universities of Shandong Province
- [21775053]
- [202201AS070020]
- [202201AU070061]
- [ZR2021YQ07]
- [ZR2020QB094]
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In this study, a highly efficient and sensitive ECL immunoassay for detecting neuron-specific enolase (NSE) was developed by linking carboxylated Ru(bpy)(3)(2+) to an iron-based metal-organic framework (NH2-MIL-88 (Fe)) and using polyaniline-intercalating vanadium oxide (PVO) nanosheets as the substrate. The NH2-MIL-88 (Fe) and PVO provided abundant amino groups that enhanced the ECL signal by catalyzing the reactions of the co-reactant S2O82-. This research offers an effective strategy for ultrasensitive detection of trace proteins using ECL immunoassays.
The preparation of highly efficient electrochemiluminescence (ECL) illuminants is an effective method to improve the sensitivity and repeatability of ECL immunoassay. In this study, we prepared an ECL immunoassay for efficient and sensitive detection of neuron-specific enolase (NSE) by linking carboxylated Ru(bpy)(3)(2+) to an iron-based metal-organic framework (NH2-MIL-88 (Fe)) via an amide bond as an ECL signal probe. NH2-MIL-88 (Fe) possesses a large number of amino groups that can catalyze the co-reactant S2O82-, which generates abundant reaction intermediates SO4 center dot- around Ru(dcbpy)(3)(2+), reduces the loss of material transport and energy transfer between SO4 center dot- and Ru(dcbpy)(3)(2+), and significantly enhances the ECL signal. We used polyaniline-intercalating vanadium oxide (PVO) nanosheets as the substrates to capture NSE owing to the large specific surface area and extraordinary conductivity of the nanosheets. Similarly, PVO nanosheets also possess abundant amino groups, which can act as co-reaction promoters to catalyze the reaction of S2O82- to SO4 center dot-, enhancing the ECL signal of the immunoassay. Therefore, we constructed a dual-enhanced ECL immunoassay with Ru (dcbpy)(3)(2+)/NH2-MIL-88 (Fe) and PVO as the signal probe and substrate, respectively, which exhibited excellent sensitivity and selectivity for detecting NSE. This study offers an effective strategy for ultrasensitive detection of trace proteins using ECL immunoassays.
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