☆ 4.8 Article

Split aptamer regulated CRISPR/Cas12a biosensor for 17β-estradiol through a gap-enhanced Raman tags based lateral ﬂow strategy

BIOSENSORS & BIOELECTRONICS (2022)

Related references

Note: Only part of the references are listed.

Article Engineering, Environmental

CRISPR-cas12a mediated SERS lateral flow assay for amplification-free detection of double-stranded DNA and single-base mutation

Yuanfeng Pang et al.

Summary: By combining CRISPR-Cas12a and SERS technology, the sensitivity and specificity of LFA nucleic acid detection have been improved, allowing for direct quantification of HIV-1 dsDNA in less than 1 hour and recognition of HIV-1 single-base drug resistance mutations. This paper-based CRISPR-SERS strip has great potential for point-of-care testing in resource-poor or non-laboratory environments.

CHEMICAL ENGINEERING JOURNAL (2022)

Add to Collection

Article Chemistry, Applied

Universal fluorescence nanoprobes to enhance the sensitivity of immunochromatographic assay for detection of 17β-estradiol in milk

Xin Lu et al.

Summary: This study focused on the detection of estrogens in the environment and food using on-site immunochromatographic assay method. They developed universal probes based on quantum dot nanobeads and recombinant protein A, achieving enhanced sensitivity for the detection system. The proposed ICA system showed promising results for the rapid and sensitive detection of small-molecule pollutants in environmental and food samples.

FOOD CHEMISTRY (2022)

Add to Collection

Article Chemistry, Applied

A fluorescence aptasensor based on carbon quantum dots and magnetic Fe3O4 nanoparticles for highly sensitive detection of 17β-estradiol

Qingyi Wei et al.

Summary: A novel fluorescent aptasensor was developed for sensitive detection of E2, showing a good linear range and low detection limit. The aptasensor exhibited high selectivity and good reproducibility for E2, suggesting its great potential for detecting food contaminants in the food industry.

FOOD CHEMISTRY (2022)

Add to Collection

Article Nanoscience & Nanotechnology

Divalent Cation Dependence Enhances Dopamine Aptamer Biosensing

Nako Nakatsuka et al.

Summary: Oligonucleotide receptors (aptamers) undergo conformational changes upon target recognition, with the new dopamine aptamer showing significant responses to Mg2+ and Ca2+ in high ionic-strength conditions. While divalent cation-associated signal amplification was observed for the new dopamine aptamer, it was not seen in the serotonin aptamer or the old dopamine aptamer, indicating allosteric binding interactions between divalent cations and dopamine for the new dopamine aptamer.

ACS APPLIED MATERIALS & INTERFACES (2021)

Add to Collection

Article Chemistry, Analytical

Ultrasensitive Detection of 17β-Estradiol (E2) Based on Multistep Isothermal Amplification

Weiya Wang et al.

Summary: A multi-step isothermal amplification fluorescence strategy was developed for quantitative detection of E2 at very low levels, with a good linear relationship between fluorescence value and E2 concentration. This method showed high sensitivity and specificity, providing a new possibility for highly sensitive detection of other small-molecule targets.

ANALYTICAL CHEMISTRY (2021)

Add to Collection

Article Chemistry, Applied

Truncated affinity-improved aptamers for 17β-estradiol determination by AuNPs-based colorimetric aptasensor

Lu Qiao et al.

Summary: Truncated aptamer E09, derived from original E00, showed improved affinity and higher specificity for E2 detection, achieving a detection limit of 0.02μg/mL using gold nanoparticles-based colorimetric method. This work demonstrates the potential application of truncated aptamers in detection of E2 and provides a proof-of-concept study for truncation of other long-sequence aptamers.

FOOD CHEMISTRY (2021)

Add to Collection

Article Engineering, Environmental

A split aptamer (SPA)-based sandwich-type biosensor for facile and rapid detection of streptomycin

Qian Zhu et al.

Summary: A sensitive EWF biosensor for STR detection using SPA has been developed, showing good selectivity and reuse capability. The detection steps are simple and the recovery rate for water samples is high.

JOURNAL OF HAZARDOUS MATERIALS (2021)

Add to Collection

Article Chemistry, Analytical

Dual-Mode Sensing Platform Guided by Intramolecular Electrochemiluminescence of a Ruthenium Complex and Cationic N,N-Bis(2-(trimethylammonium iodide)propylene) Perylene-3,4,9,10-tetracarboxydiimide for Estradiol Assay

Jingwei Xue et al.

Summary: A dual-mode sensing platform using cationic PDA+-assembled DNA strands as a quencher was suggested for estradiol detection, with improved reaction rate and ECL efficiency. This method not only avoids difficulty of assembling signal indicators but also greatly enhances sensitivity, offering high detection accuracy.

ANALYTICAL CHEMISTRY (2021)

Add to Collection

Article Nanoscience & Nanotechnology

Electrochemical Microfluidic Paper-Based Aptasensor Platform Based on a Biotin-Streptavidin System for Label-Free Detection of Biomarkers

Tao Ming et al.

Summary: The study introduces a paper-based aptasensor platform for convenient and affordable detection of biomarkers. Experimental results demonstrate its ability to accurately determine 17-beta-Estradiol in a wide concentration range and detect targets in clinical samples, showing its potential for various applications.

ACS APPLIED MATERIALS & INTERFACES (2021)

Add to Collection

Article Chemistry, Analytical

Highly Sensitive Colorimetric/Surface-Enhanced Raman Spectroscopy Immunoassay Relying on a Metallic Core-Shell Au/Au Nanostar with Clenbuterol as a Target Analyte

Lihong Su et al.

Summary: A dual-model colorimetric/SERS lateral flow immunoassay based on a metallic core-shell Au/Au nanostar was constructed for ultrasensitive determination of clenbuterol. The method achieved visual detection of clenbuterol at 5 ng/mL and quantitative detection as low as 0.05 ng/mL, significantly lower than traditional AuNPs-based immunoassays.

ANALYTICAL CHEMISTRY (2021)

Add to Collection

Article Biophysics

Electrochemical aptasensor for 17β-estradiol using disposable laser scribed graphene electrodes

Zhu Chang et al.

Summary: A novel electrochemical aptasensor for detecting 17β-estradiol (E2) was developed, showing high sensitivity and selectivity. The sensor was successfully translated from glass carbon electrodes (GCE) to low-cost, disposable laser-scribed graphene electrodes (LSGE), indicating its potential for commercial and portable detection systems. The study demonstrates the feasibility of the sensing platform for E2 and similar biological targets.

BIOSENSORS & BIOELECTRONICS (2021)

Add to Collection

Article Biophysics

Aptamer assisted CRISPR-Cas12a strategy for small molecule diagnostics

Chenqi Niu et al.

Summary: The Molecular Radar is a versatile diagnostic strategy that allows for rapid and convenient detection of small molecules using the CRISPR Cas12a system and DNA aptamer technology. This method shows potential for on-site molecular diagnostics in point-of-care settings, providing high specificity and sensitivity for target molecule detection. Additionally, the unique characteristics of CRISPR-Cas12a demonstrated in this study contribute to a better understanding of Cas12a and offer guidance for further research on CRISPR-Cas12a-based biosensors.

BIOSENSORS & BIOELECTRONICS (2021)

Add to Collection

Article Chemistry, Applied

Graphite-like carbon nitride-laden gold nanoparticles as signal amplification label for highly sensitive lateral flow immunoassay of 17β-estradiol

Xiaolin Yao et al.

Summary: A LFIA method utilizing graphite-like carbon nitride-laden gold nanoparticles was developed to enhance sensitivity for 17 beta-estradiol in foods, achieving a three-fold improvement in analytical performance compared to conventional gold nanoparticles-based LFIA. This method was successfully applied to detect estrogen in contaminated food samples, indicating great potential for its application in food products.

FOOD CHEMISTRY (2021)

Add to Collection

Article Chemistry, Analytical

Visual-afterglow dual-mode immunochromatographic strip for 17β-estradiol detection in milk

Yi Zhang et al.

Summary: Continuous intake of 17 beta-estradiol (E2) residue from animal-derived food can pose health risks to consumers, making rapid and sensitive E2 detection essential. A dual-mode ICS strategy was developed for this purpose, with an afterglow detection mode achieving a limit of detection of 0.5 ng/mL, twenty times more sensitive than the visual mode. This strategy has been successfully applied to detect estradiol in milk and validated by HPLC-FLD.

TALANTA (2021)

Add to Collection

Article Chemistry, Multidisciplinary

Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets

Ying Xiong et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2020)

Add to Collection

Article Chemistry, Analytical

LSPR-based colorimetric immunosensor for rapid and sensitive 17β-estradiol detection in tap water

Antonio Minopoli et al.

SENSORS AND ACTUATORS B-CHEMICAL (2020)

Add to Collection

Article Biotechnology & Applied Microbiology

CRISPR-Cas12-based detection of SARS-CoV-2

James P. Broughton et al.

NATURE BIOTECHNOLOGY (2020)

Add to Collection

Article Endocrinology & Metabolism

Endocrine-disrupting chemicals: implications for human health

Linda G Kahn et al.

Lancet Diabetes & Endocrinology (2020)

Add to Collection

Article Chemistry, Analytical

Engineering and Application of a Myoglobin Binding Split Aptamer

Shaoyuan Li et al.

ANALYTICAL CHEMISTRY (2020)

Add to Collection

Article Biophysics

A split aptamer sensing platform for highly sensitive detection of theophylline based on dual-color fluorescence colocalization and single molecule photobleaching

Shi Gang Liu et al.

BIOSENSORS & BIOELECTRONICS (2020)

Add to Collection

Review Chemistry, Analytical

Highly sensitive and specific detection of small molecules using advanced aptasensors based on split aptamers: A review

Xiaoyan Qi et al.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2020)

Add to Collection

Article Multidisciplinary Sciences

Electric field-driven microfluidics for rapid CRISPR-based diagnostics and its application to detection of SARS-CoV-2

Ashwin Ramachandran et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Add to Collection

Article Chemistry, Analytical

Unconventional Split Aptamers Cleaved at Functionally Essential Sites Preserve Biorecognition Capability

Ruoyu Wang et al.

ANALYTICAL CHEMISTRY (2019)

Add to Collection

Article Chemistry, Multidisciplinary

Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor

Yifan Dai et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2019)

Add to Collection

Article Chemistry, Physical

SERS-based lateral flow immunoassay of troponin I by using gap-enhanced Raman tags

Boris N. Khlebtsov et al.

NANO RESEARCH (2019)

Add to Collection

Article Chemistry, Multidisciplinary

Electron Transport Across Plasmonic Molecular Nanogaps Interrogated with Surface-Enhanced Raman Scattering

Li Lin et al.

ACS NANO (2018)

Add to Collection

Article Multidisciplinary Sciences

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

Janice S. Chen et al.

SCIENCE (2018)

Add to Collection

Article Multidisciplinary Sciences

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

Jonathan S. Gootenberg et al.

SCIENCE (2018)

Add to Collection

Article Chemistry, Multidisciplinary

A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva

Haixiang Yu et al.

CHEMICAL SCIENCE (2017)

Add to Collection

Article Chemistry, Analytical

Lateral Flow Aptasensor for Small Molecule Targets Exploiting Adsorption and Desorption Interactions on Gold Nanoparticles

Omar A. Alsager et al.

ANALYTICAL CHEMISTRY (2017)

Add to Collection

Article Nanoscience & Nanotechnology

Highly Sensitive Detection of Hormone Estradiol E2 Using Surface-Enhanced Raman Scattering Based Immunoassays for the Clinical Diagnosis of Precocious Puberty

Rui Wang et al.

ACS APPLIED MATERIALS & INTERFACES (2016)

Add to Collection

Article Chemistry, Physical

Helical Structure and Circular Dichroism Spectra of DNA: A Theoretical Study

Tomoo Miyahara et al.

JOURNAL OF PHYSICAL CHEMISTRY A (2013)

Add to Collection

© Peeref 2019-2024. All rights reserved.