4.8 Article

Engineering type I methanotrophic bacteria as novel platform for sustainable production of 3-hydroxybutyrate and biodegradable polyhydroxybutyrate from methane and xylose

Journal

BIORESOURCE TECHNOLOGY
Volume 363, Issue -, Pages -

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2022.127898

Keywords

Methylotuvimicrobium alcaliphilum 20Z; (R)-3-hydroxybutyrate; Poly (3-hydroxybutyrate); Tunable genetic module

Funding

  1. C1 Gas Refinery Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2015M3D3A1A01064882]
  2. Korea Institute of Marine Science & Technology Promotion (KIMST) - Ministry of Oceans and Fisheries, Korea [20220532]

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This study demonstrated that M. alcaliphilum 20Z can co-utilize methane and xylose from anthropogenic activities and lignocellulose biomass to produce (R)-3-hydroxybutyrate and poly (3-hydroxybutyrate). The production of (R)-3-hydroxybutyrate was optimized by introducing additional thioesterase and a tunable genetic module. The study also highlighted the importance of pathway reversibility as a design strategy for balancing driving force and intermediate accumulation.
Methylotuvimicrobium alcaliphilum 20Z recombinant strain co-utilizing methane and xylose from anthropogenic activities and lignocellulose biomass is a promising cell factory platform. In this study, the production of (R)-3-hydroxybutyrate and poly (3-hydroxybutyrate) in M. alcaliphilum 20Z was demonstrated. The production of (R)-3-hydroxybutyrate was optimized by introducing additional thioesterase, and a tunable genetic module. The final recombinant strain produced the highest titer of 334.52 +/- 2 mg/L (R)-3-hydroxybutyrate (yield of 1,853 +/- 429 mg/g dry cell weight). The poly (3-hydroxybutyrate) yielded 1.29 +/- 0.08% (w/w) from methane and xylose in one-stage cultivation. Moreover, the study demonstrated the importance of pathway reversibility as an effective design strategy for balancing the driving force and intermediate accumulation. This is the first demonstration of the production of biodegradable poly (3-hydroxybutyrate) from methane in type I methanotrophs, which is a key step toward sustainable biomanufacturing and carbon-neutral society.

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