Rapidly achieving partial denitrification from nitrate wastewater in a alkaline fermentation system with primary sludge as inoculated sludge and fermentable substrate

In order to promote practical engineering application of anaerobic ammonium oxidation(anammox) process, reduction of primary sludge(PS) in wastewater treatment plants(WWTPs) and removal of nitrate contaminant, a single-stage simultaneous alkaline fermentation coupled with partial denitrification(SAFPD) system was established successfully in this study. Nitrite production was rapidly achieved from nitrate wastewater with PS as inoculated sludge and fermentable substrate under anaerobic and anoxic operating conditions. During the stable operation period, the primary sludge reduction(PSR) and productivity of organic matters were 27.9% and 483.8mgCOD/gVSS, with nitrate removal of 90.7%, NO3- to NO2- transformation ratio(NTR) of 80.0%. After 125 days of acclimation, the relative abundance of Thauera, Dechloromonas and Candidatus_Competibacter increased from 0.17%, 0.02% and 0.05% to 11.58%, 4.28% and 5.6% respectively. Above results showed that this SAFPD system not only realized the reduction of PS and nitrate removal, but also laid a solid foundation for anammox process with its high nitrite production.

Automated summary

This study established a single-stage simultaneous alkaline fermentation coupled with partial denitrification system, achieving reduction of primary sludge and removal of nitrate. It also laid a solid foundation for anammox process with high nitrite production.