Journal
BIORESOURCE TECHNOLOGY
Volume 362, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2022.127756
Keywords
Rhizobium sp.; Heterotrophic nitrification-aerobic denitrification; Cold-resistant; Nitrogen removal; Nitrogen metabolism
Funding
- Hebei Provincial Key Research Projects [21373904D]
- Science and Technology Research Project of Higher Education in Hebei Province [ZD2017233]
- Doctoral Starting up Foundation of Hebei University of Science and Technology, China [1181374]
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In this study, a cold-resistant Rhizobium strain W57 was isolated and identified, showing significant de-nitrogenation efficiency at 15℃. The nitrogen metabolic pathways and enzyme activities were determined for strain W57. The findings suggest that W57 has potential application in cold sewage treatment.
For improving the poor de-nitrogen efficiency and effluent quality faced by wastewater treatment plants in winter, a novel cold-resistant strain, Rhizobium sp. W57 was isolated. Strain W57 presented dramatic de-nitrogen efficiencies including 98.73 % of NH4+-N, 99.98 % of NO3--N, 100 % of NO2--N and approximately 100 % of mixed nitrogen (NH4+-N and NO3--N) at 15 degrees C. Optimum parameters of W57 for aerobic denitrification were determined. Additionally, functional genes (amoA, napA, nirK, norB, and nosZ) and key enzymes (nitrate reductase and nitrite reductase) activities were determined. Nitrogen balance analysis suggested that assimilation played a dominant role in de-nitrogen by WS7, the NH4+-N metabolic pathway was deduced as NH4+-N -> NH2OH -> NO -> N2O -> N-2, and the NO3--N/NO2--N metabolic pathway was deduced as NO3--N -> NO2--N -> NO -> N2O -> N-2 . The coldresistant Rhizobium sp. W57 has great application feasibility in cold sewage treatment.
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