High Drug Loading Nanoparticles Stabilized with Autologous Serum Proteins Passively Inhibits Tumor Growth

We report drug nanocrystals stabilized with host-specific serum proteins with high loading (similar to 63% w/w). The human serum derived curcumin nanoparticles (Cur-NanoSera) showed superior in vitro anticancer efficiency compared to a free drug with substantial hemocompatibility. The preadsorbed protein coating impeded further protein corona formation, even with repeated serum exposures. Acute and subacute toxicity evaluations post single and dual injections of CS7BL/6 mice indicated that Cur-NanoSera showed no prominent inflammatory response or organ damage in the in-bred mice. Passive accumulation of Cur-NanoSera in tumor tissue significantly suppressed its growth in a syngeneic breast tumor model in addition to controlling tumor burden associated splenomegaly.

Automated summary

This study reports drug nanocrystals stabilized with host-specific serum proteins, providing high loading capacity. Human serum derived curcumin nanoparticles (Cur-NanoSera) demonstrated superior in vitro anticancer efficiency compared to free drugs and exhibited good hemocompatibility. The preadsorbed protein coating prevented further protein corona formation, even after repeated serum exposures. Acute and subacute toxicity evaluations in mice showed no prominent inflammatory response or organ damage. Passive accumulation of Cur-NanoSera in a breast tumor model significantly suppressed tumor growth and controlled splenomegaly associated with tumor burden.