Journal
BIOELECTROCHEMISTRY
Volume 147, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2022.108172
Keywords
Ovomucoid; Electrochemiluminescence; Immunosensor; Titanium dioxide; Gold nanourchins
Funding
- Universiti Brunei Darussalam [UBD/RSCH/1.4/FICBF(b)/2018/010]
- Brunei Research Council
- Ministry of Education of Brunei Darussalam
- Universiti Teknologi Brunei
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This work presents the development of an electrochemiluminescence immunosensor using a novel nanostructured composite of titanium dioxide and gold nanourchins for the detection of protein allergen Ovomucoid found in eggs. The sensor exhibited a low limit of detection, wide linear range, and excellent selectivity, reproducibility, and recovery rates in real food samples.
This work reports on the first electrochemiluminescence (ECL) immunosensor employing a novel nanostructured composite of titanium dioxide (TiO2) and gold nanourchins (AuNU) to detect protein allergen Ovomucoid (Ovm) found in eggs. TiO2 and AuNU were dispersed in Nafion and drop-casted onto SPGE with Tris(2,2'-bipyridyl)-ruthenium (II) ([Ru(bpy)(3)](2+)) and tri-n-propylamine (TPrA) served as strong luminophore/co-reactant pairs as a source of ECL signals. The linear range, limit of detection, reproducibility and practical applications of the sensor were assessed. The fabricated ECL immuosensor produced a promising limit of detection of as low as 0.01 pg/mL. Two linear ranges of 0.01-50 pg/mL and 100-750 pg/mL with corresponding correlation coefficients of R-2 = 0.99136 and R-2 = 0.97829) respectively, were determined. Despite its simple fabrication method, this label-free immunosensor also showcased excellent selectivity, reproducibility, interference-resistance and yielded outstanding recoveries between 97.42 and 104.05% of Ovm analysis in spiked real food samples.
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