4.5 Article

Kinesin-7 CENP-E is essential for chromosome alignment and spindle assembly of mouse spermatocytes

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ELSEVIER
DOI: 10.1016/j.bbamcr.2022.119306

Keywords

Kinesin-7; CENP-E; Spermatocyte; Chromosome; Spindle; Meiosis

Funding

  1. National Natural Science Foundation of China [82001608]
  2. Natural Science Foundation of Fujian Province, China [2019J05071]
  3. Fujian Provincial Health Technology Project [2018-1-69]
  4. Fujian Medical University high level talents scientific research start-up funding project [XRCZX2017025]

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This study reveals the role of CENP-E in regulating chromosome alignment and spindle organization in male spermatocyte meiotic division, contributing to faithful chromosome segregation and stability.
Genome stability depends on chromosome congression and alignment during cell division. Kinesin-7 CENP-E is critical for kinetochore-microtubule attachment and chromosome alignment, which contribute to genome stability in mitosis. However, the functions and mechanisms of CENP-E in the meiotic division of male spermatocytes remain largely unknown. In this study, by combining the use of chemical inhibitors, siRNA-mediated gene knockdown, immunohistochemistry, and high-resolution microscopy, we have found that CENP-E inhibition results in chromosome misalignment and metaphase arrest in dividing spermatocyte during meiosis. Strikingly, we have revealed that CENP-E regulates spindle organization in metaphase I spermatocytes and cultured GC-2 spd cells. CENP-E depletion leads to spindle elongation, chromosome misalignment, and chromosome instability in spermatocytes. Together, these findings indicate that CENP-E mediates the kinetochore recruitment of BubR1, spindle assembly checkpoint and chromosome alignment in dividing spermatocytes, which finally contribute to faithful chromosome segregation and chromosome stability in the male meiotic division.

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