Journal
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
Volume 1866, Issue 11, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bbagen.2022.130206
Keywords
Monoclonal antibody; Neutron scattering; Microstructure; Dynamic; Surface adsorption
Categories
Funding
- Presidential Foundation of CAEP, China [YZJJLX2018004]
- National Natural Science Foundation of China [11605171]
- Ludo Frevel Crystallography Scholarships from the International Centre for Diffraction Data (ICDD)
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This review outlines the current state of utilizing neutron scattering to investigate monoclonal antibodies (mAbs). The basic knowledge and principles of neutron scattering and mAbs are introduced, followed by specific examples of applications in structure and dynamics research. The review demonstrates and discusses how neutron scattering can be utilized to study the structures and dynamics of mAbs.
Over the past several decades, great progresses have been made for the pharmaceutical industry of monoclonal antibody (mAb). More and more mAb products were approved for human therapeutics. This review describes the state of art of utilizing neutron scattering to investigate mAbs, in the aspects of structures, dynamics, physicochemical stability, functionality, etc. Firstly, brief histories of mAbs and neutron scattering, as well as some basic knowledges and principles of neutron scattering were introduced. Then specific examples were demonstrated. For the structure and structural evolution investigation of in dilute and concentrated mAbs solution, in situ small angle neutron scattering (SANS) was frequently utilized. Neutron reflectometry (NR) is powerful to probe the absorption behaviors of mAbs on various surfaces and interfaces. While for dynamic investigation, quasi-elastic scattering techniques such as neutron spin echo (NSE) demonstrate the capabilities. With this review, how to utilize and take advantages of neutron scattering on investigating structures and dynamics of mAbs were demonstrated and discussed.
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