4.6 Article

Protein engineering of carbonyl reductase for asymmetric synthesis of ticagrelor precursor (1S)-2-chloro-1-(3,4-difluorophenyl)ethanol

Related references

Note: Only part of the references are listed.
Article Biochemistry & Molecular Biology

Directed evolution of a carbonyl reductase LsCR for the enantioselective synthesis of (1S)-2-chloro-1-(3,4-difluorophenyl) ethanol

Hua-Tao Liu et al.

Summary: A novel enzyme engineering strategy was developed in this study to modify carbonyl reductase LsCR, resulting in increased catalytic activity and efficiency.

BIOORGANIC CHEMISTRY (2022)

Article Chemistry, Physical

Controlling Stereopreferences of Carbonyl Reductases for Enantioselective Synthesis of Atorvastatin Precursor

Feng Cheng et al.

Summary: Key residues regulating the stereopreference of a carbonyl reductase from Kluyveromyces marxianus (KmCR) were identified through semi-rational engineering, leading to the successful conversion of Prelog selectivity to complete anti-Prelog selectivity. This knowledge was then used to successfully predict and engineer the stereopreferences of other CRs sharing sequence identities with KmCR.

ACS CATALYSIS (2021)

Article Chemistry, Multidisciplinary

Application of Ketoreductase in Asymmetric Synthesis of Pharmaceuticals and Bioactive Molecules: An Update (2018-2020)

Zhining Li et al.

Summary: With the advancement of biocatalysis, significant progress has been made in the asymmetric synthesis of chiral secondary alcohol intermediates catalyzed by ketoreductase, including the synthesis of chiral alcohols with one stereocenter through bioreduction and the synthesis of chiral alcohols with two contiguous stereocenters through dynamic reductive kinetic resolution.

CHEMICAL RECORD (2021)

Editorial Material Chemistry, Physical

Performance metrics for sustainable catalysis in industry

Jean-Paul Lange

Summary: This article presents simple metrics that can guide early catalysis research to promote sustainable and affordable manufacturing of fuels and chemicals. These metrics focus on resource efficiency, waste/CO2 production, selectivity, and conversion performances in the process.

NATURE CATALYSIS (2021)

Article Biotechnology & Applied Microbiology

Tuning enzymatic properties by protein engineering toward catalytic tetrad of carbonyl reductase

Feng Cheng et al.

Summary: Enzyme engineering towards catalytic-tetrad residues often leads to activity loss, but a directed evolution campaign on carbonyl reductase KmCR resulted in a beneficial residue A100, leading to variants with improved enzymatic activities. The KmCR variant KmCR_A100S exhibited increased catalytic efficiency, improved stereoselectivity, and extended substrate scope compared to the wild-type KmCR. This study's findings have universal significance to carbonyl reductase engineering.

BIOTECHNOLOGY AND BIOENGINEERING (2021)

Article Biochemical Research Methods

Fluorescence-based screening for engineered aldo-keto reductase KmAKR with improved catalytic performance and extended substrate scope

Shuai Qiu et al.

Summary: Aldo-keto reductase-catalyzed transformations of ketones to chiral alcohols have been widely used in the pharmaceutical industry. By directed evolution, the best variant M5-Q213A/T23V exhibited enhanced activity-thermostability towards (5R)-1 and strict R-stereoselectivity towards 10 substrates, showing potential for the synthesis of aliphatic/aromatic R-configuration alcohols.

BIOTECHNOLOGY JOURNAL (2021)

Article Biochemical Research Methods

QMEANDisCo-distance constraints applied on model quality estimation

Gabriel Studer et al.

BIOINFORMATICS (2020)

Article Biochemistry & Molecular Biology

Dual Substrate Specificity of the Rutinosidase fromAspergillus nigerand the Role of Its Substrate Tunnel

Katerina Brodsky et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

Article Biochemistry & Molecular Biology

SWISS-MODEL: homology modelling of protein structures and complexes

Andrew Waterhouse et al.

NUCLEIC ACIDS RESEARCH (2018)

Article Chemistry, Physical

Impact of Mutations on the Binding Pocket of Soybean Lipoxygenase: Implications for Proton-Coupled Electron Transfer

Pengfei Li et al.

JOURNAL OF PHYSICAL CHEMISTRY LETTERS (2018)

Article Chemistry, Multidisciplinary

The E factor 25 years on: the rise of green chemistry and sustainability

Roger A. Sheldon

GREEN CHEMISTRY (2017)

Article Chemistry, Applied

Development of a Practical Enzymatic Process for Preparation of (&ITS&IT)-2-Chloro-1-(3,4-difluorophenyl)ethanol

Xiang Guo et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2017)

Article Chemistry, Organic

Biocatalytic approaches to a key building block for the anti-thrombotic agent ticagrelor

Katharina G. Hugentobler et al.

ORGANIC & BIOMOLECULAR CHEMISTRY (2016)

Article Chemistry, Physical

Highly Stereoselective Biocatalytic Synthesis of Key Cyclopropane Intermediate to Ticagrelor

Kari E. Hernandez et al.

ACS CATALYSIS (2016)

Article Chemistry, Multidisciplinary

New Ways to Boost Molecular Dynamics Simulations

Elmar Krieger et al.

JOURNAL OF COMPUTATIONAL CHEMISTRY (2015)

Article Biochemistry & Molecular Biology

Characterization of a robust anti-Prelog short-chain dehydrogenase/reductase ChKRED20 from Chryseobacterium sp. CA49

Tuo-Xian Tang et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2014)

Article Cardiac & Cardiovascular Systems

Ticagrelor Increases Adenosine Plasma Concentration in Patients With an Acute Coronary Syndrome

Laurent Bonello et al.

JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY (2014)

Letter Urology & Nephrology

Ticagrelor in Clopidogrel-Resistant Patients Undergoing Maintenance Hemodialysis

Dimitrios Alexopoulos et al.

AMERICAN JOURNAL OF KIDNEY DISEASES (2012)

Article Chemistry, Medicinal

Synthesis and biological evaluation of ticagrelor derivatives as novel antiplatelet agents

Hao Zhang et al.

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS (2012)

Article Biotechnology & Applied Microbiology

Engineered Enzymes for Chemical Production

Stephan Luetz et al.

BIOTECHNOLOGY AND BIOENGINEERING (2008)

Article Biochemistry & Molecular Biology

A method for localizing ligand binding pockets in protein structures

F Glaser et al.

PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS (2006)