Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 629, Issue -, Pages 78-85Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2022.08.068
Keywords
Histone deacetylases; Embryonic development; Neurological disorders; Embryoid body; Hedgehog signaling
Categories
Funding
- JST SPRING [JPMJSP2101]
- JSPS KAKENHI [19K08264]
- MEXT-Supported Program for the Strategic Reuter Foundation at Private Universities
- Takeda Science Foundation
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HDAC8 is involved in embryoid body formation and neuronal differentiation in P19EC cells by regulating the canonical Hedgehog (Hh) signaling pathway.
Histone acetylation and deacetylation are associated with diverse biological phenomena via gene tran-scription, and histone deacetylases (HDACs) regulate protein deacetylation. HDAC8 is associated with childhood neurological disorders that develop in the uterus and may contribute to neurodevelopment. In our previous studies, we found that HDAC8 regulates neuronal differentiation in P19 pluripotent em-bryonic carcinoma cells (P19EC cells) by regulating embryoid body (EB) formation. However, the mechanism through which HDAC8 is involved in EB formation and neuronal differentiation remains unclear. Here, we show that HDAC8 regulates EB formation and neuronal differentiation by regulating the canonical Hedgehog (Hh) signaling pathway in P19EC cells. We found that HDAC8 is possibly involved in regulating the expression of the Smoothened receptor (Smo), an important receptor in ca-nonical Hh signaling, and treatment with a Smo agonist restored EB formation ability, which was reduced in HDAC8 knockout P19EC cells. Our results demonstrate that HDAC8 functions in EB formation, which is involved in the Hh signaling pathway that is important for embryonic development. (c) 2022 Elsevier Inc. All rights reserved.
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