Journal
ARTIFICIAL ORGANS
Volume 47, Issue 3, Pages 481-489Publisher
WILEY
DOI: 10.1111/aor.14419
Keywords
decellularization; heart-lung scaffolds; tissue engineering
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Through ex vivo perfusion and decellularization, we have successfully created bioengineered transplantable heart-lung scaffolds. The development of this technology could provide a life-saving conduit, significantly reducing the risks of heart-lung surgery and improving postoperative quality of life.
Background Bioengineered transplantable heart-lung scaffolds could be potentially lifesaving in a large number of congenital and acquired cardiothoracic disorders including terminal heart-lung disease. Methods We decellularized heart-lung organ-blocs from rats (n = 10) by coronary and tracheal perfusion with ionic detergents in a modified Langendorff circuit. Results In the present project, we were able to achieve complete decellularization of the heart-lung organ-bloc. Decellularized heart-lung organ-blocs lacked intracellular components but maintained structure of the cellular walls with collagen and elastic fibers. Conclusions We present a novel model of combined perfusion and decellularization of heart-lung organ-blocs. This model is the first step on the pathway to creating bioengineered transplantable heart-lung scaffolds. We believe that further development of this technology could provide a life-saving conduit, significantly reducing the risks of heart-lung failure surgery and improving postoperative quality of life.
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