Complete genome sequence of patchouli chlorosis-associated cytorhabdovirus, a new cytorhabdovirus infecting patchouli plants in Brazil

A cytorhabdovirus, tentatively named patchouli chlorosis-associated cytorhabdovirus (PCaCV), was identified in a patchouli plant, using high-throughput sequencing, and its genome sequence was confirmed by Sanger sequencing. The PCaCV genome consists of 12,913 nucleotides and contains six open reading frames in the order 3'-N-V-P-P3-M-(G)-L-5'. The glycoprotein gene was found to contain stop codons in the coding frame; hence, this gene is considered defective. PCaCV is most closely related to tomato yellow mottle-associated virus, sharing 61.1% nucleotide sequence identity in the complete genome and 73.9% amino acid sequence identity in the L protein. These data suggest that PCaCV should be considered a new member of the genus Cytorhabdovirus, and the binomial species name Cytorhabdovirus patchoulii is proposed.

Automated summary

A cytorhabdovirus, tentatively named patchouli chlorosis-associated cytorhabdovirus (PCaCV), was identified in a patchouli plant using high-throughput sequencing. The genome sequence of PCaCV was confirmed and found to contain six open reading frames. PCaCV is most closely related to tomato yellow mottle-associated virus and should be considered a new member of the genus Cytorhabdovirus.