Oil and hypoxia alter DNA methylation and transcription of genes related to neurological function in larval Cyprinodon variegatus

DNA methylation is an important epigenetic mark involved in modulating transcription. While multiple studies document the ability of environmental stressors to alter methylation patterns, there is little information regarding the effects of oil and hypoxia on the methylome. Oil and hypoxic stress are threats in coastal ecosystems, which act as nursery habitats for developing fish. To explore the methylation altering effects of oil and hypoxia on developing fish, we exposed larval Cyprinodon variegatus to oil, hypoxia, or both for 48 h followed by 48 h of depuration in clean, normoxic conditions. We then used immunoprecipitation coupled with highthroughput sequencing (MeDIP seq) to evaluate genome-wide methylation changes. We also performed RNA seq to associate methylation and altered transcription. Oil and hypoxia together elicited greater impacts to methylation than either stressor individually. Additionally, the oil+hypoxia treatment exhibited an overlap between differentially methylated regions and differential gene expression at 20 loci. Functional analyses of these loci revealed enrichment of processes related to neurological function and development. Two neurological genes (slc1a2, asxl2) showed altered methylation of promoter CpG islands and transcriptional changes, suggesting epigenetic modulation of gene expression. Our results suggest a possible mechanism explaining altered behavior patterns noted in fish following oil exposure.

Automated summary

DNA methylation is an important epigenetic mark involved in modulating transcription. The effects of oil and hypoxia on the methylome and the subsequent impact on fish development and behavior in coastal ecosystems have been studied. The results suggest that oil and hypoxia together have greater impacts on methylation than either stressor individually. Functional analyses showed that neurological processes and development were enriched in the differentially methylated regions and differentially expressed genes. Two neurological genes exhibited altered methylation and transcriptional changes, indicating epigenetic modulation of gene expression. The study provides insights into the mechanisms underlying altered behavior patterns in fish following oil exposure.