4.5 Article

Unravelling the characterization of hypoxia-inducible factor-1α (HIF-1α) and antioxidant enzymes in clam Cyclina sinensis in response to hypoxia

Journal

AQUACULTURE RESEARCH
Volume 53, Issue 17, Pages 5937-5945

Publisher

WILEY
DOI: 10.1111/are.16062

Keywords

antioxidant enzymes; clone; Cyclina sinensis; HIF-1 alpha; hypoxia

Categories

Funding

  1. National key research and development projects [2020YFD0900203-02]

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The study shows that clams use HIF-1 alpha gene and antioxidant enzymes to cope with acute hypoxia exposure, which helps increase their adaptation to hypoxic conditions.
Aquatic hypoxia has negative or detrimental effects on clams. To cope with hypoxia stress, a complex series of physiological and biochemical strategies can be employed in clams. Hypoxia-inducible factor-1 alpha (HIF-1 alpha) and antioxidant enzymes are commonly found in aquatic animals as an adaptation against hypoxia. In the present study, we cloned the HIF-1 alpha sequence in clam Cyclina sinensis, and it consists of 2654 base pairs (bp) carrying a single open-reading frame that encompasses 2235 bp of the coding region. The predicted C. sinensis HIF-1 alpha protein (744 amino acids) has the conserved HLH-PAS domain, which contained one N-terminal basic helix-loop-helix (bHLH) domain and two Per-ARNT-Sim A/B (PAS) domains, followed by PAS-associated C-terminal (PAC) domain. HIF-1 alpha is expressed predominantly in gills. Exposed to hypoxia, the transcript level of HIF-1 alpha was significantly up-regulated in response to hypoxia, and then, the transcript level declined continuously with the time elapsed. The antioxidant enzymes (SOD, CAT and GSH-PX) in gills were increased initially and then decreased after acute hypoxia exposure. Our study indicates that the HIF-1 alpha gene and antioxidant enzymes may play an important role in cope with acute hypoxia exposure.

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