Journal
APPLIED OPTICS
Volume 61, Issue 32, Pages 9334-9341Publisher
Optica Publishing Group
DOI: 10.1364/AO.474991
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Funding
- Ministry of Science and Technology, Taiwan
- [102-2221-E-002-032-MY3]
- [108-2221-E-002-081-MY3]
- [111-2221-E-002-034-MY3]
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The mass and concentration of hemoglobin per erythrocyte are important hematological parameters. Measuring these parameters requires the value of specific refraction-index increment (RII) of oxy-genated hemoglobin, which diverges in the literature. Refractive indices of hemoglobin solutions are measured directly by digital holographic microscopy and the differences between hemoglobin from thalassemic patients and healthy volunteers are not significant.
The mass and concentration of hemoglobin per erythrocyte are important hematological parameters. Measuring these parameters from intact erythrocytes requires the value of specific refraction-index increment (RII) of oxy-genated hemoglobin, which diverges in the literature. Refractive indices of hemoglobin solutions are measured directly by digital holographic microscopy on a microfluidic channel filled with hemoglobin solutions prepared by hemolysis of fresh human erythrocytes and refractive-index standards sequentially. Hemoglobin extracted from thalassemic patients shows 3-4% higher RII than that from healthy volunteers, but the difference is not significant in comparison to inter-subject variations within each group. The quantified RIIs are applied to quantify mean corpuscular hemoglobin mass of blood from 37 human subjects, and results are in accord with standard clinical test results. (c) 2022 Optica Publishing Group
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