Candida albicans CHK1 gene regulates its cross-kingdom interactions with Streptococcus mutans to promote caries

The cross-kingdom interactions between Candida albicans and Streptococcus mutans have played important roles in early childhood caries (ECC). However, the key pathways of C. albicans promoting the cariogenicity of S. mutans are still unclear. Here, we found that C. albicans CHK1 gene was highly upregulated in their dual-species biofilms. C. albicans chk1 Delta/Delta significantly reduced the synergistical growth promotion, biofilm formation, and exopolysaccharides (EPS) production of S. mutans, the key cariogenic agent, compared to C. albicans wild type (WT) and CHK1 complementary strains. C. albicans WT upregulated the expressions of S. mutans EPS biosynthesis genes gtfB, gtfC, and gtfD, and their regulatory genes vicR and vicK, but chk1 Delta/Delta had no effects. Both C. albicans WT and chk1 Delta/Delta failed to promote the biofilm formation and EPS production of S. mutans Delta vicK and antisense-vicR strains, indicating that C. albicans CHK1 upregulated S. mutans vicR and vicK to increase the EPS biosynthesis gene expression, then enhanced the EPS production and biofilm formation to promote the cariogenicity. In rat caries model, the coinfection with chk1 Delta/Delta and S. mutans decreased the colonization of S. mutans and developed less caries especially the severe caries compared to that from the combinations of S. mutans with C. albicans WT, indicating the essential role of C. albicans CHK1 gene in the development of dental caries. Our study for the first time demonstrated the key roles of C. albicans CHK1 gene in dental caries and suggested that it may be a practical target to reduce or treat ECC.

Automated summary

This study demonstrated that Candida albicans CHK1 gene plays a crucial role in the development of dental caries by promoting the growth, biofilm formation, and exopolysaccharides production of Streptococcus mutans. The upregulation of S. mutans vicR and vicK genes by C. albicans CHK1 gene enhances the expression of EPS biosynthesis genes, leading to increased EPS production and biofilm formation. In a rat caries model, coinfection with chk1 Delta/Delta and S. mutans resulted in decreased colonization of S. mutans and less severe caries compared to other combinations.