4.6 Article

Heterologous Assembly of the Type VI Secretion System Empowers Laboratory Escherichia coli with Antimicrobial and Cell Penetration Capabilities

Related references

Note: Only part of the references are listed.
Review Biotechnology & Applied Microbiology

Metabolic engineering and synthetic biology for isoprenoid production in Escherichia coli and Saccharomyces cerevisiae

Govinda R. Navale et al.

Summary: Isoprenoids, also known as terpenoids, are diverse organic compounds found abundantly in nature, with various applications in pharmaceutical, nutraceutical, and chemical industries. Recent advancements in metabolic engineering have enabled the production of isoprenoids in heterologous host systems like Escherichia coli and Saccharomyces cerevisiae. Challenges and strategies for scale-up and engineering of isoprenoids in heterologous host systems have been discussed in detail.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2021)

Review Biochemistry & Molecular Biology

Activity, delivery, and diversity of Type VI secretion effectors

Dukas Jurenas et al.

Summary: The bacterial T6SS system secretes a variety of toxic effectors that can affect other bacteria or cells through different mechanisms. These effectors can disrupt the cell envelope, affect DNA integrity, cell division, or deplete energy resources, helping bacteria dominate their niche.

MOLECULAR MICROBIOLOGY (2021)

Article Multidisciplinary Sciences

Killing of Gram-negative and Gram-positive bacteria by a bifunctional cell wall-targeting T6SS effector

Nguyen-Hung Le et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

Article Microbiology

VgrG-dependent effectors and chaperones modulate the assembly of the type VI secretion system

Xiaoye Liang et al.

Summary: The study on Aeromonas dhakensis strain SSU reveals that the specificity of effectors is dictated by the VgrG tail, and deletion of effector-specific chaperones severely impairs T6SS secretion. The research also shows that chimeric VgrG-effector pairs can bypass the requirement of heterologous VgrG complex and effector-stuffing inside the baseplate complex is crucial for T6SS assembly.

PLOS PATHOGENS (2021)

Article Biotechnology & Applied Microbiology

Characterization of Lysozyme-Like Effector TseP Reveals the Dependence of Type VI Secretion System (T6SS) Secretion on Effectors in Aeromonas dhakensis Strain SSU

Xiaoye Liang et al.

Summary: The Type VI Secretion System (T6SS) is utilized by Gram-negative bacteria for interspecies interaction. This study identified a lysozyme-like effector TseP in Aeromonas dhakensis, showcasing its cell wall-lysing activities and T6SS dependency. Inactivating TseP and two other effectors led to the creation of a detoxified T6SS-active strain for potential protein delivery to various recipient cells.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2021)

Article Biochemistry & Molecular Biology

Engineering a customizable antibacterial T6SS-based platform in Vibrio natriegens

Biswanath Jana et al.

Summary: A customizable, modular, and inducible antibacterial toxin delivery platform was constructed in this study, by engineering a type VI secretion system (T6SS) to transform Vibrio natriegens into an effective antibacterial weapon. The platform's delivered effector repertoire, and thus toxicity range, can be easily manipulated and tested, serving as a promising foundation for novel antibacterial bio-treatments and a unique tool for studying antibacterial toxins.

EMBO REPORTS (2021)

Article Microbiology

Engineered Type Six Secretion Systems Deliver Active Exogenous Effectors and Cre Recombinase

Steven J. Hersch et al.

Summary: Genetic editing has been revolutionized by delivering Cre recombinase as a protein through engineered bacterial type six secretion system (T6SS). The versatile T6SS system can also deliver other exogenous effectors, like TseC, enhancing its efficacy against specific pathogens. This engineered system shows promise in microbiome manipulation and as a next-generation antimicrobial tool.
Article Engineering, Biomedical

Exploiting interbacterial antagonism for microbiome engineering

Sung Sun Yim et al.

Summary: Interbacterial antagonism plays a significant role in shaping microbiome assembly and stability, offering opportunities to modulate microbial communities through direct cell-to-cell contact or secreted biomolecules. Manipulating microbial communities through negative interactions is essential for understanding complex microbiome processes and properties, as well as for developing new applications in microbiome engineering.

CURRENT OPINION IN BIOMEDICAL ENGINEERING (2021)

Article Multidisciplinary Sciences

Intramolecular chaperone-mediated secretion of an Rhs effector toxin by a type VI secretion system

Tong-Tong Pei et al.

NATURE COMMUNICATIONS (2020)

Article Multidisciplinary Sciences

A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing

Beverly Y. Mok et al.

NATURE (2020)

Article Multidisciplinary Sciences

TssA-TssM-TagA interaction modulates type VI secretion system sheath-tube assembly in Vibrio cholerae

Maria Silvina Stietz et al.

NATURE COMMUNICATIONS (2020)

Review Microbiology

Assembly and Subcellular Localization of Bacterial Type VI Secretion Systems

Jing Wang et al.

ANNUAL REVIEW OF MICROBIOLOGY, VOL 73 (2019)

Review Microbiology

Citrobacter rodentium-host-microbiota interactions: immunity, bioenergetics and metabolism

Caroline Mullineaux-Sanders et al.

NATURE REVIEWS MICROBIOLOGY (2019)

Article Multidisciplinary Sciences

An onboard checking mechanism ensures effector delivery of the type VI secretion system in Vibrio cholerae

Xiaoye Liang et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2019)

Article Multidisciplinary Sciences

Efficient inter-species conjugative transfer of a CRISPR nuclease for targeted bacterial killing

Thomas A. Hamilton et al.

NATURE COMMUNICATIONS (2019)

Review Biotechnology & Applied Microbiology

Bacterial type III secretion system as a protein delivery tool for a broad range of biomedical applications

Fang Bai et al.

BIOTECHNOLOGY ADVANCES (2018)

Review Biotechnology & Applied Microbiology

Escherichia coli as a host for metabolic engineering

Sammy Pontrelli et al.

METABOLIC ENGINEERING (2018)

Review Microbiology

Pseudomonas syringae: what it takes to be a pathogen

Xiu-Fang Xin et al.

NATURE REVIEWS MICROBIOLOGY (2018)

Article Medicine, General & Internal

Vibrio spp. infections

Craig Baker-Austin et al.

NATURE REVIEWS DISEASE PRIMERS (2018)

Article Microbiology

The type VI secretion system deploys antifungal effectors against microbial competitors

Katharina Trunk et al.

NATURE MICROBIOLOGY (2018)

Article Biochemistry & Molecular Biology

Cryo-EM reconstruction of Type VI secretion system baseplate and sheath distal end

Sergey Nazarov et al.

EMBO JOURNAL (2018)

Article Biochemistry & Molecular Biology

Domestication of a housekeeping transglycosylase for assembly of a Type VI secretion system

Yoann G. Santin et al.

EMBO REPORTS (2017)

Article Multidisciplinary Sciences

Vibrio cholerae type 6 secretion system effector trafficking in target bacterial cells

Brian T. Ho et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2017)

Article Multidisciplinary Sciences

In situ architecture, function, and evolution of a contractile injection system

Desiree Bock et al.

SCIENCE (2017)

Article Biotechnology & Applied Microbiology

Microbial Herd Protection Mediated by Antagonistic Interaction in Polymicrobial Communities

Megan J. Q. Wong et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2016)

Review Microbiology

Aeromonas dhakensis, an Increasingly Recognized Human Pathogen

Po-Lin Chen et al.

FRONTIERS IN MICROBIOLOGY (2016)

Review Biology

Type VI secretion system: secretion by a contractile nanomachine

Marek Basler

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES (2015)

Article Multidisciplinary Sciences

Identification of divergent type VI secretion effectors using a conserved chaperone domain

Xiaoye Liang et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Biochemical Research Methods

Engineering Escherichia coli into a Protein Delivery System for Mammalian Cells

Analise Z. Reeves et al.

ACS SYNTHETIC BIOLOGY (2015)

Article Microbiology

A Type VI Secretion-Related Pathway in Bacteroidetes Mediates Interbacterial Antagonism

Alistair B. Russell et al.

CELL HOST & MICROBE (2014)

Review Microbiology

A View to a Kill: The Bacterial Type VI Secretion System

Brian T. Ho et al.

CELL HOST & MICROBE (2014)

Article Biochemistry & Molecular Biology

The VgrG Proteins Are a la Carte Delivery Systems for Bacterial Type VI Effectors

Abderrahman Hachani et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2014)

Article Biochemistry & Molecular Biology

Tit-for-Tat: Type VI Secretion System Counterattack during Bacterial Cell-Cell Interactions

Marek Basler et al.

Article Biochemistry & Molecular Biology

Lytic Activity of the Vibrio cholerae Type VI Secretion Toxin VgrG-3 Is Inhibited by the Antitoxin TsaB

Teresa M. Brooks et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2013)

Article Biochemistry & Molecular Biology

Haemolysin Coregulated Protein Is an Exported Receptor and Chaperone of Type VI Secretion Substrates

Julie M. Silverman et al.

MOLECULAR CELL (2013)

Article Biochemistry & Molecular Biology

ClpV recycles VipA/VipB tubules and prevents non-productive tubule formation to ensure efficient type VI protein secretion

Nicole Kapitein et al.

MOLECULAR MICROBIOLOGY (2013)

Article Multidisciplinary Sciences

Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors

Alistair B. Russell et al.

NATURE (2013)

Article Multidisciplinary Sciences

PAAR-repeat proteins sharpen and diversify the type VI secretion system spike

Mikhail M. Shneider et al.

NATURE (2013)

Article Multidisciplinary Sciences

Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae

Tao G. Dong et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

Article Multidisciplinary Sciences

Rhs proteins from diverse bacteria mediate intercellular competition

Sanna Koskiniemi et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

Review Biotechnology & Applied Microbiology

Bacterial expression systems for recombinant protein production: E. coli and beyond

Rachel Chen

BIOTECHNOLOGY ADVANCES (2012)

Article Multidisciplinary Sciences

Type VI secretion requires a dynamic contractile phage tail-like structure

M. Basler et al.

NATURE (2012)

Article Biochemical Research Methods

Fiji: an open-source platform for biological-image analysis

Johannes Schindelin et al.

NATURE METHODS (2012)

Review Microbiology

Maintaining network security: how macromolecular structures cross the peptidoglycan layer

Edie M. Scheurwater et al.

FEMS MICROBIOLOGY LETTERS (2011)

Article Multidisciplinary Sciences

Type VI secretion delivers bacteriolytic effectors to target cells

Alistair B. Russell et al.

NATURE (2011)

Article Microbiology

A Type VI Secretion System of Pseudomonas aeruginosa Targets, a Toxin to Bacteria

Rachel D. Hood et al.

CELL HOST & MICROBE (2010)

Article Biochemistry & Molecular Biology

Control of effector export by the Pseudomonas aeruginosa type III secretion proteins PcrG and PcrV

Pei-Chung Lee et al.

MOLECULAR MICROBIOLOGY (2010)

Article Biochemistry & Molecular Biology

Remodelling of VipA/VipB tubules by ClpV-mediated threading is crucial for type VI protein secretion

Gabriele Boenemann et al.

EMBO JOURNAL (2009)

Review Microbiology

Type VI secretion: a beginner's guide

Lewis E. H. Bingle et al.

CURRENT OPINION IN MICROBIOLOGY (2008)

Article Multidisciplinary Sciences

A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae

D. Ewen Cameron et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2008)

Article Multidisciplinary Sciences

Type VI secretion system translocates a phage tail spike-like protein into target cells where it cross-links actin

Stefan Pukatzki et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)

Article Biochemistry & Molecular Biology

Type VI secretion is a major virulence determinant in Burkholderia mallei

Mark A. Schell et al.

MOLECULAR MICROBIOLOGY (2007)

Article Multidisciplinary Sciences

A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus

Joseph D. Mougous et al.

SCIENCE (2006)

Article Multidisciplinary Sciences

Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system

S Pukatzki et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)

Review Immunology

Human infection with Photorhabdus asymbiotica:: an emerging bacterial pathogen

J Gerrard et al.

MICROBES AND INFECTION (2004)

Article Biotechnology & Applied Microbiology

DNA cloning by homologous recombination in Escherichia coli

YM Zhang et al.

NATURE BIOTECHNOLOGY (2000)