Nuclear Cell-Free DNA Predicts Adverse Events After Pediatric Cardiothoracic Surgery

BACKGROUND Preoperative risk stratification in cardiac surgery includes patient and procedure factors that are used in clinical decision-making. Despite these tools, unidentified factors contribute to variation in outcomes. Identification of latent physiologic risk factors may strengthen predictive models. Nuclear cell-free DNA (ncfDNA) increases with tissue injury and drops to baseline levels rapidly. The goal of this investigation is to measure and to observe ncfDNA kinetics in children undergoing heart operations with cardiopulmonary bypass (CPB), linking biomarkers, organ dysfunction, and outcomes. METHODS This is a prospective observational study of 116 children <18 years and >3 kg undergoing operations with CPB. Plasma ncfDNA samples were collected and processed in a stepwise manner at predefined perioperative time points. The primary outcome measure was occurrence of postoperative cardiac arrest or extracorporeal membrane oxygenation. RESULTS Data were available in 116 patients (median age, 0.9 years [range, 0-17.4 years]; median weight, 7.8 kg [range, 3.2-98 kg]). The primary outcome was met in 6 of 116 (5.2%). Risk of primary outcome was 2% with ncfDNA <20 ng/mL and 33% with ncfDNA >20 ng/mL (odds ratio, 25; CI, 3.96-158; P [.001). Elevated ncfDNA was associated with fewer hospital-free days (P <.01). CONCLUSIONS This study analyzes ncfDNA kinetics in children undergoing operations with CPB for congenital heart disease. Elevated preoperative ncfDNA is strongly associated with postoperative arrest and extracorporeal membrane oxygenation. Further studies are needed to validate this technology as a tool to predict morbidity in children after cardiac surgical procedures. (c) 2023 by The Society of Thoracic Surgeons. Published by Elsevier Inc.

Automated summary

This study investigates the kinetics of nuclear cell-free DNA (ncfDNA) in children undergoing cardiac surgery with cardiopulmonary bypass (CPB). The results show that elevated preoperative ncfDNA levels are strongly associated with postoperative cardiac arrest and extracorporeal membrane oxygenation. Further research is needed to validate this technology as a predictive tool for morbidity in children after cardiac surgical procedures.