4.7 Article

Effects of TAMP family on the tight junction strand network and barrier function in epithelial cells

Journal

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
Volume 1517, Issue 1, Pages 234-250

Publisher

WILEY
DOI: 10.1111/nyas.14889

Keywords

marvelD3; occludin; TAMP; tight junctions; TJ strand; tricellulin

Funding

  1. Nakatomi Foundation
  2. Kazato Research Foundation
  3. Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering
  4. Japan Society for the Promotion of Science [16H06280, 18K15085, 18K06223, 21K06156]

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MarvelD3 does not affect the morphology of TJ strands and barrier function in MDCK II cells. The carboxy-terminal cytoplasmic tail of tricellulin is dispensable for barrier improvement.
Occludin, tricellulin, and marvelD3 belong to the tight junction (TJ)-associated MARVEL protein family. Occludin and tricellulin jointly contribute to TJ strand branching point formation and epithelial barrier maintenance. However, whether marvelD3 has the same function remains unclear. Furthermore, the roles of the carboxy-terminal cytoplasmic tail, which is conserved in occludin and tricellulin, on the regulation of TJ strand morphology have not yet been explored in epithelial cells. We established tricellulin/occludin/marveld3 triple-gene knockout (tKO) MDCK II cells and evaluated the roles of marvelD3 in the TJ strand structure and barrier function using MDCK II cells and a mathematical model. The complexity of TJ strand networks and paracellular barrier did not change in tKO cells compared to that in tricellulin/occludin double-gene knockout (dKO) cells. Exogenous marvelD3 expression in dKO cells did not increase the complexity of TJ strand networks and epithelial barrier tightness. The expression of the carboxy-terminal truncation mutant of tricellulin restored the barrier function in the dKO cells, whereas occludin lacking the carboxy-terminal cytoplasmic tail was not expressed on the plasma membrane. These data suggest that marvelD3 does not affect the morphology of TJ strands and barrier function in MDCK II cells and that the carboxy-terminal cytoplasmic tail of tricellulin is dispensable for barrier improvement.

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