Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 305, Issue -, Pages 200-208Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2015.11.041
Keywords
Acid mine drainage (AMD); Municipal wastewater (MWW); Sulfidogenic bioreactors; Kinetics modelling; Phylogenetic analysis
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Funding
- Office Of The Director
- Office of Integrative Activities [1458952] Funding Source: National Science Foundation
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The kinetics and microbial ecology in sulfidogenic bioreactors used in a novel two-stage process for co-treatment of acid mine drainage (AMD) and municipal wastewater (MWW) were investigated. Michaelis-Menten modeling of COD oxidation by sulfate reducing bacteria (SRB) (V-max = 0.33 mg L-1 min(-1), K-m = 4.3 mg L-1) suggested that the V-max, can be reasonably achieved given the typical COD values in MWW and anticipated mixing with AMD. Non-competitive inhibition modeling (K-i = 6.55 mg L-1) indicated that excessive iron level should be avoided to limit its effects on SRB. The COD oxidation rate was positively correlated to COD/sulfate ratio and SRB population, as evidenced by dsrA gene copies. Phylogenetic analysis revealed diverse microbial communities dominated by sulfate reducing delta-proteobacteria. Microbial community and relative quantities of SRB showed significant differences under different COD/sulfate ratios (0.2, 1 and 2), and the highest dsrA gene concentration and most complex microbial diversity were observed under COD/sulfate ratio 2. Major species were associated with Desulfovirga, Desulfobulbus, Desulfovibrio, and Syntrophus sp. The reported COD kinetics, SRB abundances and the phylogenetic profile provide insights into the co-treatment process and help identify the parameters of concerns for such technology development. (C) 2015 Elsevier B.V. All rights reserved.
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