Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 304, Issue -, Pages 474-480Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jhazmat.2015.11.016
Keywords
Microcystin-LR (MC-LR); Aptamer; Calorimetric sensor; High sensitivity; High selectivity
Categories
Funding
- National Natural Science Foundation of China (NSFC) [21277099, 21477085, 21307091, 51208367]
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A simple and highly sensitive aptamer-based colorimetric sensor was developed for selective detection of Microcystin-LR (MC-LR). The aptamer (ABA) was employed as recognition element which could bind MC-LR with high-affinity, while gold nanoparticles (AuNPs) worked as sensing materials whose plasma resonance absorption peaks red shifted upon binding of the targets at a high concentration of sodium chloride. With the addition of MC-LR, the random coil aptamer adsorbed on Au NPs altered into regulated structure to form MC-LR-aptamer complexes and broke away from the surface of Au NPs, leading to the aggregation of AuNPs, and the color converted from red to blue due to the interparticle plasmon coupling. Results showed that our aptamer-based calorimetric sensor exhibited rapid and sensitive detection performance for MC-LR with linear range from 0.5 nM to 7.5 mu M and the detection limit reached 0.37 nM. Meanwhile, the pollutants usually coexisting with MC-LR in pollutant water samples had not demonstrated disturbance for detecting of MC-LR. The mechanism was also proposed suggesting that high affinity interaction between aptamer and MC-LR significantly enhanced the sensitivity and selectivity for MC-LR detection. Besides, the established method was utilized in analyzing real water samples and splendid sensitivity and selectivity were obtained as well. (C) 2015 Elsevier B.V. All rights reserved.
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